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Mfg Part Number

Ab02382-44.0

Vector Labs Ab02382-44.0 Anti-SARS-CoV-2 and SARS-CoV nucleocapsid [mBG17], Cat IgM, kappa (50 μg)

VECTOR LABS

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Quick Overview

The original mouse IgG1 version of this antibody was raised by immunizing BALB/c mice with a recombinant SARS-Cov-2 N protein corresponding to AA133-419.

$692.90

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Description

Product Class: Purified
Clone ID: mBG17
Synonyms: NP; nucleoprotein; NC; Protein N; Nucleocapsid protein; SARS-CoV-2 Protein N; SARS-CoV-2 Nucleocapsid protein; SARS Coronavirus; SARS-CoV-2; SARS CoV 2; 2019-nCoV; 17; SARS-CoV nucleocapsid
Amount: 50 μg
Application Codes Clone: ELISA; WB; IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric cat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: mBG17 strongly recognizes SARS-CoV-2 N protein and SARS-CoV N protein. To lesser extent, it cross-reacts with the HuNL63 N protein. Western blot analysis with truncated fragments of N protein suggested that its epitope resides in AA381-419 at the C-terminal.
Application Notes: Specificty of the original mouse IgG1 version of this antibody to SARS-CoV-2 and SARS-Cov was confirmed by Specificty of the original mouse IgG1 version of this antibody to SARS-CoV-2 and SARS-Cov was confirmed by ELISA (Terry et al., 2021; pmid: 33714753). This antibody detected SARS-Cov-2 N protein in Western blot exhibiting labeling patterns suggesting a linear epitope (Terry et al., 2021; pmid: 33714753). mBG17 was shown to be very effective in staining SARS-Cov-2 N protein in immunofluorescence in SARS-Cov-2-infected Vero cells, both methanol and paraformaldehyde fixed (Terry et al., 2021; pmid: 33714753). Finally, the original version of mBG17 was demonstrated to be an effective detection antibody in a sandwich ELISA assay suggesting it's potential use in SARS-Cov-2 diagnostics (Terry et al., 2021; pmid: 33714753).

Product Class:	Purified
Clone ID:	mBG17
Synonyms:	NP; nucleoprotein; NC; Protein N; Nucleocapsid protein; SARS-CoV-2 Protein N; SARS-CoV-2 Nucleocapsid protein; SARS Coronavirus; SARS-CoV-2; SARS CoV 2; 2019-nCoV; 17; SARS-CoV nucleocapsid
Amount:	50 μg
Application Codes Clone:	ELISA; WB; IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric cat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	mBG17 strongly recognizes SARS-CoV-2 N protein and SARS-CoV N protein. To lesser extent, it cross-reacts with the HuNL63 N protein. Western blot analysis with truncated fragments of N protein suggested that its epitope resides in AA381-419 at the C-terminal.
Application Notes:	Specificty of the original mouse IgG1 version of this antibody to SARS-CoV-2 and SARS-Cov was confirmed by Specificty of the original mouse IgG1 version of this antibody to SARS-CoV-2 and SARS-Cov was confirmed by ELISA (Terry et al., 2021; pmid: 33714753). This antibody detected SARS-Cov-2 N protein in Western blot exhibiting labeling patterns suggesting a linear epitope (Terry et al., 2021; pmid: 33714753). mBG17 was shown to be very effective in staining SARS-Cov-2 N protein in immunofluorescence in SARS-Cov-2-infected Vero cells, both methanol and paraformaldehyde fixed (Terry et al., 2021; pmid: 33714753). Finally, the original version of mBG17 was demonstrated to be an effective detection antibody in a sandwich ELISA assay suggesting it's potential use in SARS-Cov-2 diagnostics (Terry et al., 2021; pmid: 33714753).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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