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Mfg Part Number

Ab02383-44.0

Vector Labs Ab02383-44.0 Anti-SARS-CoV-2 nucleocapsid [mBG86], Cat IgM, kappa (50 μg)

VECTOR LABS

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Quick Overview

The original mouse IgG1 version of this antibody was raised by immunizing BALB/c mice with a recombinant SARS-Cov-2 N protein corresponding to AA133-419.

$692.90

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Description

Product Class: Purified
Clone ID: mBG86
Synonyms: NP; nucleoprotein; NC; Protein N; Nucleocapsid protein; SARS-CoV-2 Protein N; SARS-CoV-2 Nucleocapsid protein; SARS Coronavirus; SARS-CoV-2; SARS CoV 2; 2019-nCoV; 86
Amount: 50 μg
Application Codes Clone: ELISA; WB; IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric cat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: mBG86 is specific to SARS-CoV-2 N protein and does not react with the SARS-CoV N protein or other similar N proteins (such as MERS-CoV, HuCoV-OC43, HuCoV-NL63, HuCoV-HKU1 and HuCoV-229E N). Western blot analysis with truncated fragments of N protein suggested that its epitope resides in AA133-179.
Application Notes: Specificty of the original mouse IgG1 version of this antibody to SARS-CoV-2 was confirmed by ELISA (Terry et al., 2021; pmid: 33714753). mBG86 is a particularly useful antibody as it can be used to detect SARS-Cov-2 N protein and differentiate it from SARS-CoV. Western blot analysis demonstrated that this antibody exhibits two band labeling, suggesting a specificity for conformational epitopes on the N protein (Terry et al., 2021; pmid: 33714753). This antibody was shown to detect SARS-CoV-2 N protein in methanol-stained Vero cells by immunofluorescence (Terry et al., 2021; pmid: 33714753). Finally, the original version of mBG86 was demonstrated to be an effective detection antibody in a sandwich ELISA assay suggesting it's potential use in SARS-Cov-2 diagnostics (Terry et al., 2021; pmid: 33714753).

Product Class:	Purified
Clone ID:	mBG86
Synonyms:	NP; nucleoprotein; NC; Protein N; Nucleocapsid protein; SARS-CoV-2 Protein N; SARS-CoV-2 Nucleocapsid protein; SARS Coronavirus; SARS-CoV-2; SARS CoV 2; 2019-nCoV; 86
Amount:	50 μg
Application Codes Clone:	ELISA; WB; IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric cat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	mBG86 is specific to SARS-CoV-2 N protein and does not react with the SARS-CoV N protein or other similar N proteins (such as MERS-CoV, HuCoV-OC43, HuCoV-NL63, HuCoV-HKU1 and HuCoV-229E N). Western blot analysis with truncated fragments of N protein suggested that its epitope resides in AA133-179.
Application Notes:	Specificty of the original mouse IgG1 version of this antibody to SARS-CoV-2 was confirmed by ELISA (Terry et al., 2021; pmid: 33714753). mBG86 is a particularly useful antibody as it can be used to detect SARS-Cov-2 N protein and differentiate it from SARS-CoV. Western blot analysis demonstrated that this antibody exhibits two band labeling, suggesting a specificity for conformational epitopes on the N protein (Terry et al., 2021; pmid: 33714753). This antibody was shown to detect SARS-CoV-2 N protein in methanol-stained Vero cells by immunofluorescence (Terry et al., 2021; pmid: 33714753). Finally, the original version of mBG86 was demonstrated to be an effective detection antibody in a sandwich ELISA assay suggesting it's potential use in SARS-Cov-2 diagnostics (Terry et al., 2021; pmid: 33714753).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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