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Mfg Part Number

Ab02385-1.1

Vector Labs Ab02385-1.1 Anti-1-pHis [SC50-3], Mouse IgG1, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original rabbit version of this antibody was raised by immunizing rabbits with a 1-pTza library conjugated to the carrier protein keyhole limpet hemocyanin (KLH).

$517.40

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Description

Product Class: Purified
Clone ID: SC50-3
Synonyms: 1-phosphohistidine; N1-Phosphohistidine; histidine
Amount: 200 μg
Application Codes Clone: WB; ELISA; Immunoaffinity purification
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Rabbit IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for 1-pHis and does not cross react with phosphotyrosine (pTyr) or the other pHis isomers.
Application Notes: This antibody can be used for the identification of 1-pHis substrates and functional study of pHis. A dot blot was preformed on rabbit antisera, the 1-pTza, 3-pTza peptide libraries, His control library and a pTyr peptide with this antibody demonstrating its specificity to 1-pHis (Fuhs et al, 2015; pmid:26140597). This antibody has been also used for a western blot on His6-NDPK-A protein (Felix et al, 2020; pmid:32384736). Immunofluorescence was preformed on mouse tissues with this antibody (Yang et al, 2019; pmid:31721359). An ELISA was preformed on 3-day differentiated Sca1+-VPCs with this antibody (Yang et al, 2019; pmid:31721359). Ca1+-VPCs were lysed and immunoprecipitated with this clone (Yang et al, 2019; pmid:31721359). Finally, immunoaffinity purification (IAP) columns, containing protein A crosslinked with this antibody. The samples were SK-N-BE(2) xenograft tumors from mice (Adam et al, 2020; pmid:32392889).

Product Class:	Purified
Clone ID:	SC50-3
Synonyms:	1-phosphohistidine; N1-Phosphohistidine; histidine
Amount:	200 μg
Application Codes Clone:	WB; ELISA; Immunoaffinity purification
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Rabbit IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for 1-pHis and does not cross react with phosphotyrosine (pTyr) or the other pHis isomers.
Application Notes:	This antibody can be used for the identification of 1-pHis substrates and functional study of pHis. A dot blot was preformed on rabbit antisera, the 1-pTza, 3-pTza peptide libraries, His control library and a pTyr peptide with this antibody demonstrating its specificity to 1-pHis (Fuhs et al, 2015; pmid:26140597). This antibody has been also used for a western blot on His6-NDPK-A protein (Felix et al, 2020; pmid:32384736). Immunofluorescence was preformed on mouse tissues with this antibody (Yang et al, 2019; pmid:31721359). An ELISA was preformed on 3-day differentiated Sca1+-VPCs with this antibody (Yang et al, 2019; pmid:31721359). Ca1+-VPCs were lysed and immunoprecipitated with this clone (Yang et al, 2019; pmid:31721359). Finally, immunoaffinity purification (IAP) columns, containing protein A crosslinked with this antibody. The samples were SK-N-BE(2) xenograft tumors from mice (Adam et al, 2020; pmid:32392889).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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