Mfg Part Number
Ab02386-23.0
Vector Labs Ab02386-23.0 Anti-3-pHis [SC56-2], Rabbit IgG, kappa (200 μg)
Quick Overview
The original rabbit version of this antibody was raised by immunizing rabbits with a 3-pTza library conjugated to the carrier protein keyhole limpet hemocyanin (KLH).
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | SC56-2 |
| Synonyms: | 3-phosphohistidine; N3-Phosphohistidine; histidine |
| Amount: | 200 μg |
| Application Codes Clone: | IAC; WB; IF; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for 3-pHis and does not cross react with phosphotyrosine (pTyr) or the other pHis isomers. |
| Application Notes: | This antibody can be used for the identification of 3-pHis substrates and functional study of pHis. It was used in an immunoblot performed on four recombinant HK proteins—EnvZ, WalK, ArcB, and EvgS (Kinoshita-Kikuta et al, 2020; pmid:32360198). SC56-2 was also used in Western blot analyses on NDPK-B and PGAM5 proteins (Panda et al, 2016; pmid:27453048) and on mouse liver tissue as well as LX-2 cells (Gong et al, 2020; pmid:32860079). Furthermore, immunoaffinity purification was preformed using this antibody on SK-N-BE(2) xenograft tumors from mice (Adam et al, 2020; pmid:32392889). This clone was also utilized in the immunofluorescence staining (Yang et al, 2019; pmid:31721359). Finally, an ELISA was preformed on 3-day differentiated Sca1+-VPCs with this antibody (Yang et al, 2019; pmid:31721359). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |