Mfg Part Number
Ab02387-1.29
Vector Labs Ab02387-1.29 Anti-3-pHis [SC39-6], Mouse Fab fragment, His-Tagged, kappa (100 μg)
Quick Overview
The original rabbit version of this antibody was raised by immunizing rabbits with a 3-pTza library conjugated to the carrier protein keyhole limpet hemocyanin (KLH).
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| Product Class: | Purified |
|---|---|
| Clone ID: | SC39-6 |
| Heavy Chain Modification: | His-Tagged |
| Synonyms: | 3-phosphohistidine; N3-Phosphohistidine; histidine |
| Amount: | 100 μg |
| Application Codes Clone: | WB; IAP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric Fab mouse fragment was made using the variable domain sequences of the original Rabbit IgG format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for 3-pHis and does not cross react with phosphotyrosine (pTyr) or the other pHis isomers. |
| Application Notes: | This antibody can be used for the identification of 3-pHis substrates and functional study of pHis. SC39-6 can be used to detect 3-pHis via Western blot (Fuhs et al, 2015; pmid: 26140597; Hunter et al., 2019). This clone was also used for phosphoenrichment using immunoaffinity purification (Hunter et al., 2019). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |