Mfg Part Number
Ab02388-23.0
Vector Labs Ab02388-23.0 Anti-3-pHis [SC44-1], Rabbit IgG, kappa (200 μg)
Quick Overview
The original rabbit version of this antibody was raised by immunizing rabbits with a 3-pTza library conjugated to the carrier protein keyhole limpet hemocyanin (KLH).
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| Product Class: | Purified |
|---|---|
| Clone ID: | SC44-1 |
| Synonyms: | 3-phosphohistidine; N3-Phosphohistidine; histidine |
| Amount: | 200 μg |
| Application Codes Clone: | WB; IAP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for 3-pHis and does not cross react with phosphotyrosine (pTyr) or the other pHis isomers. |
| Application Notes: | This antibody can be used for the identification of 3-pHis substrates and functional study of pHis. It was shown to detect various proteins containing 3-pHis, such as SCS and ACLY (Fuhs et al, 2015; pmid: 26140597). SC44-1 was also used for phosphoenrichment using immunoaffinity purification (Hunter et al., 2019). This clone was used to analyse the prevalence of 3-pHis in hepatocellular carcinoma by Western blot (Hindupur et al., 2018; pmid: 29562234). In the same study, SC44-1 was further utilized to demonstrate that LHPP is a protein histidine phosphatase (Hindupur et al., 2018; pmid: 29562234). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |