Mfg Part Number
Ab02389-10.3
Vector Labs Ab02389-10.3 Anti-Tau [MN423], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
The original antibody was generated by hyper-immunizing C3H mice with tangle-enriched fragments of the Alzheimer paired helical filaments core.
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| Product Class: | Purified |
|---|---|
| Clone ID: | MN423 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | MAPT; TAU; PHF-tau; MAPTL; MTBT1; TAU; PHF; Tau proteins; Alzheimer paired helical filaments; Paired helical filament-tau; Microtubule-associated protein tau; Neurofibrillary tangle protein; mAb 423; NOAL 6.423; NOAL 6/66.423.2 |
| Amount: | 200 μg |
| Application Codes Clone: | WB; IHC; IF; ICC; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This is a reformatted chimeric human IgG1 Fc Silent™ antibody made using the variable domain sequences of the original mouse IgG2b format for improved compatibility with existing reagents, assays, and techniques. |
| Specificity Statement: | This antibody is specific for the pronase-resistant core PHF and the 12 kDa tau fragment. It does not recognize normal full-length tau. It is also reported that this antibody specifically recognizes tau truncated at Glu391 on the C terminus side. The addition or removal of a single residue at the C-terminus abolishes immunoreactivity. |
| Application Notes: | The original format of this antibody was characterized in detail (Novak et al., 1989; PMID: 2602435) and has been extensively utilized in Alzheimer's disease research: It played a crucial role in the structural characterization of the Alzheimer paired helical filament (PHF) core (Wischik et al., 1988; PMID: 2455299), and was employed for extracting two peptide fragments (9.5 and 12 kDa) of the PFH pronase-resistant core through immunoblotting (Wischik et al. 1988; PMID: 3132715). Additionally, it was used to study the folding of the Alzheimer’s core PHF subunit through competitive ELISA and western blotting (Skrabana et al., 2004; PMID: 15196943). It has been instrumental in the molecular investigation of neurofibrillary degeneration in Alzheimer's disease (Bondareff et al., 1994; PMID: 7509849). Epitope mapping revealed that it requires a glycine at position -3 with respect to the C terminus (Khuebachova et al., 2002; PMID: 11983234). In the realm of tau protein studies related to Alzheimer's disease, it has been employed for immunohistochemistry on brain tissue samples (Mondragón-Rodríguez et al., 2014; PMID: 24033439). Double and triple-labeled immunofluorescence was conducted using this antibody on brain tissue from Alzheimer's disease patients (Mondragón-Rodríguez et al., 2014; PMID: 24033439) (García-Sierra et al., 2003; PMID: 12719624). This antibody was used to immunoblot purified candidate tau analogs (Novak et al., 1993; PMID: 7679073). Furthermore, it was employed in immunohistochemical staining of brain tissue from patients with Alzheimer's disease, NCI, PSP, or CBD (Berry et al., 2004; PMID: 15475684). Western blot analysis on brain tissue from Alzheimer's patients was performed (Fitzpatrick et al., 2017; PMID: 28678775). This antibody was also used for a competitive ELISA on the Alzheimer's disease expression cDNA library made from the hippocampus of a 70-year-old patient who died from Alzheimer’s disease (Khuebachova et al., 2002; PMID: 11983234). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |