Mfg Part Number
Ab02397-1.1
Vector Labs Ab02397-1.1 Anti-LDL receptor [12G4], Mouse IgG1, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing mice with a peptide corresponding to amino acid residues 195-222 (CDGGPDCKDKSDEENCAVATCRPDEFQC) of the human LDL receptor.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 12G4 |
| Synonyms: | LDLR; Low density lipoprotein receptor |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC; IHC; block |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody recognizes and binds an epitope between amino acid residues 195-222 of the human low density lipoprotein receptor. |
| Application Notes: | The binding specificity of this antibody was detected using ELISA. This antibody is reported to be used to identify LDL-R expression level in cancer cell lines like HepG2, HeLa, MCF-7, Jurkat, Ramos, HuH7, A549, MDA-MB-231 and Hek293 using flow cytometry. It was found that HepG2 and HeLa cells express LDL-R most strongly. This antibody is also reported to cross react with mouse, rat and hamster LDL receptors. It strongly cross reacts with C2C12, CHO-K1, and YB2/0 cells. 12G4 antibody binding site is the same as that of LDLs and it competes with LDLs for binding site on A549 cells. This antibody was also used for the immunohistochemical analysis of LDL-receptors on cells from the colon, testes, pancreas, gastric mucosa, the basal cell layer of the buccal and exocervical mucosa, and the various renal tubules. It was also used for immunohistochemical analysis of mammary adenocarcinomas (US20090175789). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |