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Mfg Part Number

Ab02397-23.0

Vector Labs Ab02397-23.0 Anti-LDL receptor [12G4], Rabbit IgG, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing mice with a peptide corresponding to amino acid residues 195-222 (CDGGPDCKDKSDEENCAVATCRPDEFQC) of the human LDL receptor.

$517.40

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Description

Product Class: Purified
Clone ID: 12G4
Synonyms: LDLR; Low density lipoprotein receptor
Amount: 200 μg
Application Codes Clone: ELISA; FC; IHC; block
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognizes and binds an epitope between amino acid residues 195-222 of the human low density lipoprotein receptor.
Application Notes: The binding specificity of this antibody was detected using ELISA. This antibody is reported to be used to identify LDL-R expression level in cancer cell lines like HepG2, HeLa, MCF-7, Jurkat, Ramos, HuH7, A549, MDA-MB-231 and Hek293 using flow cytometry. It was found that HepG2 and HeLa cells express LDL-R most strongly. This antibody is also reported to cross react with mouse, rat and hamster LDL receptors. It strongly cross reacts with C2C12, CHO-K1, and YB2/0 cells. 12G4 antibody binding site is the same as that of LDLs and it competes with LDLs for binding site on A549 cells. This antibody was also used for the immunohistochemical analysis of LDL-receptors on cells from the colon, testes, pancreas, gastric mucosa, the basal cell layer of the buccal and exocervical mucosa, and the various renal tubules. It was also used for immunohistochemical analysis of mammary adenocarcinomas (US20090175789).

Product Class:	Purified
Clone ID:	12G4
Synonyms:	LDLR; Low density lipoprotein receptor
Amount:	200 μg
Application Codes Clone:	ELISA; FC; IHC; block
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognizes and binds an epitope between amino acid residues 195-222 of the human low density lipoprotein receptor.
Application Notes:	The binding specificity of this antibody was detected using ELISA. This antibody is reported to be used to identify LDL-R expression level in cancer cell lines like HepG2, HeLa, MCF-7, Jurkat, Ramos, HuH7, A549, MDA-MB-231 and Hek293 using flow cytometry. It was found that HepG2 and HeLa cells express LDL-R most strongly. This antibody is also reported to cross react with mouse, rat and hamster LDL receptors. It strongly cross reacts with C2C12, CHO-K1, and YB2/0 cells. 12G4 antibody binding site is the same as that of LDLs and it competes with LDLs for binding site on A549 cells. This antibody was also used for the immunohistochemical analysis of LDL-receptors on cells from the colon, testes, pancreas, gastric mucosa, the basal cell layer of the buccal and exocervical mucosa, and the various renal tubules. It was also used for immunohistochemical analysis of mammary adenocarcinomas (US20090175789).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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