Mfg Part Number
Ab02403-1.1
Vector Labs Ab02403-1.1 Anti-Notch 1 [WC75], Mouse IgG1, lambda (200 μg)
Quick Overview
The original antibody was isolated panning against phage display libraries generated from BMV (bone marrow), CS (spleen) and DP47 (frame work based CS) cells.
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| Product Class: | Purified |
|---|---|
| Clone ID: | WC75 |
| Synonyms: | Notch1; hN1; TAN-1; Neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1 |
| Amount: | 200 μg |
| Application Codes Clone: | IP; FC; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody recognizes and binds the Notch negative regulatory region (NRR) of Notch 1. |
| Application Notes: | This antibody is capable of immunoprecipitating Notch 1 but not Notch 4. The binding affinity of this antibody towards Notch1 was checked using ELISA. The binding of this antibody to full length Notch 1 overexpressed on HEK cells was confirm using flow cytometry. Flow cytometry was also used to study the expression of Notch 1 on cancer cell lines like LS-1034, TALL-1, BxPC3, and Colo_205. This antibody does not compete with DLL4 for binding site on Notch1. The original human IgG1 version of this antibody is capable of inhibiting Jag2 mediated Notch1 signalling with an EC50 value of 0.13±0.09 nM. This antibody partially inhibited Hes1 transactivation. WC75 also inhibited the proliferation of NOTCH1 mutated T-ALL cell lines such as DND41 and KOPT-K1 (PMID: 20161710). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |