Mfg Part Number
Ab02404-23.0
Vector Labs Ab02404-23.0 Anti-Notch 1 [WC613], Rabbit IgG, lambda (200 μg)
Quick Overview
The original antibody was isolated panning against phage display libraries generated from BMV (bone marrow), CS (spleen) and DP47 (frame work based CS) cells.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | WC613 |
| Synonyms: | Notch1; hN1; TAN-1; Neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1 |
| Amount: | 200 μg |
| Application Codes Clone: | IP; FC; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognizes and binds the ligand-binding region (LBD) of Notch 1. |
| Application Notes: | This antibody is capable of immunoprecipitating Notch 1 but not Notch 4. The binding affinity of this antibody towards Notch1 was checked using ELISA. The binding of this antibody to full length Notch 1 overexpressed on HEK cells was confirm using flow cytometry. Flow cytometry was also used to study the expression of Notch 1 on cancer cell lines like LS-1034, TALL-1, BxPC3, and Colo_205. The antagonistic activity of WC613 was strongly dependent on the particular ligand-expressing cell line used to activate Notch1. This antibody competes with DLL4 for binding to Notch1. This antibody is capable of inhibiting Jag2 mediated Notch1 signalling with an EC50 value of 5±3 nM. This antibody completely inhibited Hes1 transactivation (PMID: 20161710). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |