Vector Labs Ab02405-2.3 Anti-Notch 1 [23814], Mouse IgG2a, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 23814
Heavy Chain Modification: Fc Silent™
Synonyms: Notch1; hN1; TAN-1; Neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1
Amount: 200 μg
Application Codes Clone: FC; Block; Therapeutic; SPR
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognizes and binds both human and mouse notch 1 protein. This antibody does not cross react with other members of the notch family like notch 2 or notch 3. This antibody binds the ligand-binding domain of the Notch1 receptor that specifically blocks ligand-induced activation.
Application Notes: Surface plasmon resonance confirmed that the original human IgG1 version of antibody 23814 binds the extracellular domain of the human and mouse notch 1 with an affinity of Kd= 2.3 nM and 3.7 nM respectively. Flow cytometric analysis confirmed that 23814 specifically bound only human and mouse notch 1 expressed on surface of CHO cells, but not notch 2 or notch 3. This antibody blocked Notch1 function in vivo, inhibited functional angiogenesis, and inhibited tumor growth without causing gastrointestinal toxicity. The lack of toxicity allowed for combination of 23814 and the VEGFR inhibitor tivozanib, resulting in significant growth inhibition of several VEGFR inhibitor-resistant tumor models. Monotherapy with 23814 antibody resulted in potent tumor growth inhibition of Ki-168 PDX tumors. Addition of 23814 to tivozanib treatment resulted in significantly greater tumor necrosis, as well as increased induction of hypoxia genes, compared with tivozanib monotherapy (PMID: 25995436).

Product Class:	Purified
Clone ID:	23814
Heavy Chain Modification:	Fc Silent™
Synonyms:	Notch1; hN1; TAN-1; Neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1
Amount:	200 μg
Application Codes Clone:	FC; Block; Therapeutic; SPR
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognizes and binds both human and mouse notch 1 protein. This antibody does not cross react with other members of the notch family like notch 2 or notch 3. This antibody binds the ligand-binding domain of the Notch1 receptor that specifically blocks ligand-induced activation.
Application Notes:	Surface plasmon resonance confirmed that the original human IgG1 version of antibody 23814 binds the extracellular domain of the human and mouse notch 1 with an affinity of Kd= 2.3 nM and 3.7 nM respectively. Flow cytometric analysis confirmed that 23814 specifically bound only human and mouse notch 1 expressed on surface of CHO cells, but not notch 2 or notch 3. This antibody blocked Notch1 function in vivo, inhibited functional angiogenesis, and inhibited tumor growth without causing gastrointestinal toxicity. The lack of toxicity allowed for combination of 23814 and the VEGFR inhibitor tivozanib, resulting in significant growth inhibition of several VEGFR inhibitor-resistant tumor models. Monotherapy with 23814 antibody resulted in potent tumor growth inhibition of Ki-168 PDX tumors. Addition of 23814 to tivozanib treatment resulted in significantly greater tumor necrosis, as well as increased induction of hypoxia genes, compared with tivozanib monotherapy (PMID: 25995436).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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