Mfg Part Number
Ab02406-1.1
Vector Labs Ab02406-1.1 Anti-Notch 1 [N248A], Mouse IgG1, lambda (200 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with recombinant notch 1 fusion protein as immunogen. The fusion protein contained N-terminal leader peptide, EGF-like repeats 35 to 36, NRR (including Lin A, B and C domains and the HD domain) and a small portion of intracellular sequence.
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| Product Class: | Purified |
|---|---|
| Clone ID: | N248A |
| Synonyms: | Notch1; hN1; TAN-1; Neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; Block |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody binds to notch 1 at two distinct sets of epitopes, one in Lin-A domain and other in HD-C domain (aa 1705- 1710), causing the notch 1 to be locked down in the silent conformation, and thus preventing the receptor from being activated by its ligands. |
| Application Notes: | This antagonist antibody binds notch 1 with an affinity of Kd= 0.33 nM. The binding epitopes of Notch 1 antibody was analyzed by domain swap and ELISA binding assays. mAb N248A inhibits both T-cell acute lymphoblastic leukemia (T-ALL) and breast cancer cell growth in cell culture. N248A also inhibits T-cell lymphoblastic leukemia in murine xenograft tumor model (WO2012080891). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |