Mfg Part Number
Ab02431-10.0
Vector Labs Ab02431-10.0 Anti-PD-L1 [AB1], Human IgG1, lambda (200 μg)
Quick Overview
The original antibody was generated from a yeast display library by panning against B7-H1-Fc biotinylated protein.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | AB1 |
| Synonyms: | CD274; PDL1; B7-H1; B7H1; B7 homolog 1; hPD-L1; PDCD1L1; PDCD1LG1; PDCD1 ligand 1; Programmed cell death 1 ligand 1; Programmed death ligand 1 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, chimeric human antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds human PD-L1. |
| Application Notes: | The scFv version of this antibody binds human B7-H1 with an affinity of 2.88 nM. The ability of this antibody to block binding of PD-1-Fc protein or B7.1-Fc protein to B7-H1-Fc was evaluated using ELISA. This antibody could effectively block B7-H1/PD-1 binding and B7-H1/B7.1 binding with an IC50 value of 1.37µg/ml and 0.521.37µg/ml respectively. The ability of the AB1 to bind to human B7-H1, mouse B7-H1 was determined by flow cytometric analysis. This antibody did not exhibit any binding to 293F cells transfected with mouse B7-H1, human PD-1 or human ICOS. This antibody was also shown to IFN-γ release in a PBMC asssay. It was also reported that this antibody can inhibit A375 human melanoma tumor growth in mouse model (WO2015195163). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |