Vector Labs Ab02442-3.0 Anti-VCAM-1 [6C7.1], Mouse IgG2b, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 6C7.1
Synonyms: Vascular cell adhesion protein 1; Vcam1; V-CAM 1; CD106
Amount: 200 μg
Application Codes Clone: Therapeutic; IF; FC; IHC; blocking
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for murine VCAM-1. VCAM-1 is an important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Application Notes: The original rat version of this antibody was used to treat experimental autoimmune encephalomyelitis (EAE in female SJL/N mice). It resulted in a later onset of tEAE (Engelhardt et al, 1998; pmid:9854045). To asses α4-integrin–mediated T cell interaction with VCAM-1 on blood-brain barrier–endothelium in experimental autoimmune encephalomyelitis flow cytometry was preformed on T cell blasts labeled with the rat version of this antibody (Vajkoczy et al, 2001; pmid:11518729). Futhermore, PLP-specific T lymphoblasts were used for immunofluorescence with the rat version of this antibody (Vajkoczy et al, 2001; pmid:11518729). And finally, mice were injected with the rat version of this antibody to asses how it can slow down progression of EAE. It showed an ability to slow EAE down (Vajkoczy et al, 2001; pmid:11518729). In research on EAE, T line cells were incubated with the rat version of this antibody. This was then used for flow cytometry (Laschinger and Engelhardt, 200; pmid:10626664). To evaluate vascular luminal VCAM-1 expression in NOD and Blab/c mice the rat version of this antibody was injected in mice after which they were killed and the tissue analyzed (Xu et al, 2003; pmid:12756264). VCAM-1 expression in the dermal and pial microvasculatures was quantified using immunofluorescence with the rat version of this antibody (Norman et al, 2008; pmid:18426970). To investigate the influence of VCAM-1 on tumor progression, the rat version of this antibody was used for blocking of VCAM-1 (Dieterich et al, 2019; pmid:31344266). The rat version of this antibody was also utilized for immunofluorescence on mouse retinal microvascular endothelial cells (Liu et al, 2017; pmid:28931763).

Product Class:	Purified
Clone ID:	6C7.1
Synonyms:	Vascular cell adhesion protein 1; Vcam1; V-CAM 1; CD106
Amount:	200 μg
Application Codes Clone:	Therapeutic; IF; FC; IHC; blocking
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for murine VCAM-1. VCAM-1 is an important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Application Notes:	The original rat version of this antibody was used to treat experimental autoimmune encephalomyelitis (EAE in female SJL/N mice). It resulted in a later onset of tEAE (Engelhardt et al, 1998; pmid:9854045). To asses α4-integrin–mediated T cell interaction with VCAM-1 on blood-brain barrier–endothelium in experimental autoimmune encephalomyelitis flow cytometry was preformed on T cell blasts labeled with the rat version of this antibody (Vajkoczy et al, 2001; pmid:11518729). Futhermore, PLP-specific T lymphoblasts were used for immunofluorescence with the rat version of this antibody (Vajkoczy et al, 2001; pmid:11518729). And finally, mice were injected with the rat version of this antibody to asses how it can slow down progression of EAE. It showed an ability to slow EAE down (Vajkoczy et al, 2001; pmid:11518729). In research on EAE, T line cells were incubated with the rat version of this antibody. This was then used for flow cytometry (Laschinger and Engelhardt, 200; pmid:10626664). To evaluate vascular luminal VCAM-1 expression in NOD and Blab/c mice the rat version of this antibody was injected in mice after which they were killed and the tissue analyzed (Xu et al, 2003; pmid:12756264). VCAM-1 expression in the dermal and pial microvasculatures was quantified using immunofluorescence with the rat version of this antibody (Norman et al, 2008; pmid:18426970). To investigate the influence of VCAM-1 on tumor progression, the rat version of this antibody was used for blocking of VCAM-1 (Dieterich et al, 2019; pmid:31344266). The rat version of this antibody was also utilized for immunofluorescence on mouse retinal microvascular endothelial cells (Liu et al, 2017; pmid:28931763).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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