Vector Labs Ab02445-23.0 Anti-4-1BB [BBK2], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: BBK2
Synonyms: CD137; MGC2172; Tumor necrosis factor receptor superfamily member 9; TNFRSF9; T cell antigen ILA; Interleukin activated receptor; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA; BBK-2
Amount: 200 μg
Application Codes Clone: antagonist; IHC; IP; IF; FC; blocking
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for human CD137.
Application Notes: An in vitro T cell killing assay was performed using this antibody (US20200407440). Immunofluorescence was performed on KM-H2 cells using this antibody (Ho et al, 2013; pmid:23204227). Flow cytometry was performed with this antibody on cell lines: KM-H2, L-428, L-1236 KM-H2-control, KM-H2-CD137 and stable cell lines expressing CD137 or CD137L (Ho et al, 2013; pmid:23204227). Immunoprecipitation was performed with this antibody on the cell lines: KM-H2, L-428, L-1236 KM-H2-control, KM-H2-CD137 and stable cell lines expressing CD137 or CD137L (Ho et al, 2013; pmid:23204227). This antibody was used for immunohistochemistry on human tissue from lung, liver, colon, soft tissue, thyroid gland, stomach, parathyroid gland, and spleen, parotid gland, heart, aorta, kidney, and lymph node (Broll et al, 2001; pmid:11293902). Human lymph node was used for immunohistochemistry using this antibody (Pauly et al, 2002; pmid:12101260). Flow cytometry was performed on cell line FDC-H1, Human peripheral blood mononuclear cells and B lymphocytes using this antibody (Pauly et al, 2002; pmid:12101260). This antibody was used for immunohistochemistry on human tissue (Drenkard et al, 2007; pmid:17167064). Blocking of the CD137-fc protein was tested in MoDC cells using this antibody (Lippert et al, 2008; pmid:18395851).

Product Class:	Purified
Clone ID:	BBK2
Synonyms:	CD137; MGC2172; Tumor necrosis factor receptor superfamily member 9; TNFRSF9; T cell antigen ILA; Interleukin activated receptor; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA; BBK-2
Amount:	200 μg
Application Codes Clone:	antagonist; IHC; IP; IF; FC; blocking
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for human CD137.
Application Notes:	An in vitro T cell killing assay was performed using this antibody (US20200407440). Immunofluorescence was performed on KM-H2 cells using this antibody (Ho et al, 2013; pmid:23204227). Flow cytometry was performed with this antibody on cell lines: KM-H2, L-428, L-1236 KM-H2-control, KM-H2-CD137 and stable cell lines expressing CD137 or CD137L (Ho et al, 2013; pmid:23204227). Immunoprecipitation was performed with this antibody on the cell lines: KM-H2, L-428, L-1236 KM-H2-control, KM-H2-CD137 and stable cell lines expressing CD137 or CD137L (Ho et al, 2013; pmid:23204227). This antibody was used for immunohistochemistry on human tissue from lung, liver, colon, soft tissue, thyroid gland, stomach, parathyroid gland, and spleen, parotid gland, heart, aorta, kidney, and lymph node (Broll et al, 2001; pmid:11293902). Human lymph node was used for immunohistochemistry using this antibody (Pauly et al, 2002; pmid:12101260). Flow cytometry was performed on cell line FDC-H1, Human peripheral blood mononuclear cells and B lymphocytes using this antibody (Pauly et al, 2002; pmid:12101260). This antibody was used for immunohistochemistry on human tissue (Drenkard et al, 2007; pmid:17167064). Blocking of the CD137-fc protein was tested in MoDC cells using this antibody (Lippert et al, 2008; pmid:18395851).

Specifications

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Brand	Vector Labs
UNSPSC	12161500

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