Mfg Part Number
Ab02447-2.3
Vector Labs Ab02447-2.3 Anti-Alpha Actinin 4 [Ab01AA4], Mouse IgG2a, Fc Silent™, kappa (200 μg)
Quick Overview
The original antibody was raised by immunizing mice with human breast cancer cell line SKBR3.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | Ab01AA4 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Non-muscle alpha-actinin 4 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; IHC; IP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody specific for an ALPHA-ACTININ-4 antigen, including the heavy chain CDR antigen binding site amino acid sequences CDR1, CDR2, and CDR3. |
| Application Notes: | A protein obtained using this antibody is digested with trypsin and analyzed by MALDI-MS. From the analysis it was determined that this protein was alpha-actinin-4 (US9040018). An 8% Tris-glycine gel shows recombinant alpha-actinin-4 protein, and soluble alpha-actinin-4 protein from KATO-3 conditioned cell medium isolated with this antibody (US9040018). ELISA was performed using this antibody on plasma samples (Control: plasma from healthy people, Breast Cancer: plasma from breast cancer patients) from control and patient groups (US9040018). Immunohistochemistry is performed with this antibody using a Actinin-4 IHC Kit on a total of 714 breast tumor and control samples (US9040018). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |