Mfg Part Number
Ab02452-10.0
Vector Labs Ab02452-10.0 Anti-B7-H4 [6H3], Human IgG1, kappa (200 μg)
Quick Overview
The original antibody was raised by immunizing a DO11.10 mouse with B7-H4.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | 6H3 |
| Synonyms: | B7H4; B7 homolog 4; B7h.5; O8E; Immune costimulatory protein B7-H4; B7S1; Protein B7S1; B7x; T-cell costimulatory molecule B7x; VTCN1; V-set domain-containing T-cell activation inhibitor 1 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC; IHC; functional assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the IgC region of human B7-H4. |
| Application Notes: | Humanized variants of this antibody 6H3V6 and 6H3V7 are available on request. This antibody is able to neutralize the effect of an B7-H4 blocking antibody (US9574000). Flow cytometry was performed on HEK293 cells that expressed full length human B7-H4 using this antibody (US9574000). This antibody induces robust internalization of B7-H4 expressed on the surface of both the B7-H4 transfected cell line and the B7-H4 positive breast cancer cell line SK-BR-3 (US9574000). Immunohistochemistry was performed on human tissue using this antibody (US9574000). An internalization assay was performed using this antibody on EG7 (B7-H4 negative cells), EG7.IG7 (B7-H4 low expressing cells), and EG7.IVB3 (B7-H4 high expressing cells) (US9574000). Antibody-Dependent Cell-Mediated Cytotoxicity assay was performed on EG7.hB7-H4 (clone IVB3) cells and SK-BR-3 cells using this antibody (US9574000). An ELISA was performed on B7-H4ECD his-tagged protein using this antibody (US9574000). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |