Mfg Part Number
Ab02457-10.3

Vector Labs Ab02457-10.3 Anti-HDGF [H3], Human IgG1, Fc Silent™, kappa (200 μg)

Quick Overview
The original antibody was raised by immunizing mice with full-length recombinant HDGF. This antibody binds a different epitope on HDGF than antibody C1.
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Product Class:Purified
Clone ID:H3
Heavy Chain Modification:Fc Silent™
Synonyms:Hepatoma-derived growth factor; High mobility group protein 1-like 2; HMG-1L2
Amount:200 μg
Application Codes Clone:IP; IF; IHC; neutralizing; WB
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody is specific for the HDGF protein.
Application Notes:This antibody was used for immunoprecipitation on total cell lysates from H460 NSCLC cells (US20070243191). The neutralizing ability of this antibody was tested on A549. The result was a significant decrease in tumor size (US20070243191). This antibody was fluorescently labelled and injected into nude mice to test the antibody distribution. The antibodies evenly distributed in the animals including the tumors. This antibody entered into the cytoplasmic compartment of some tumor cells (US20070243191). Immunohistochemistry with this antibody was performed on tissues from NSCLC patients (US20070243191). Western blot was performed on HDGF protein using this antibody (Ren et al, 2009;pmid:19435872). ELISA was performed on the HDGF protein using this antibody (US20070243191).
More Information
Brand Vector Labs
UNSPSC 12161500