Mfg Part Number
Ab02458-23.0
Vector Labs Ab02458-23.0 Anti-HDGF [C1], Rabbit IgG, kappa (200 μg)
Quick Overview
The original antibody was raised by immunizing mice with full-length recombinant HDGF. This antibody binds a different epitope on HDGF than antibody H3.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | C1 |
| Synonyms: | Hepatoma-derived growth factor; High mobility group protein 1-like 2; HMG-1L2 |
| Amount: | 200 μg |
| Application Codes Clone: | IP; IF; IHC; neutralizing; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the HDGF protein. |
| Application Notes: | This antibody was used for immunoprecipitation on total cell lysates from H460 NSCLC cells (US20070243191). The neutralizing ability of this antibody was tested on A549. The result was a significant decrease in tumor size (US20070243191). This antibody was fluorescently labelled and injected into nude mice to test the antibody distribution. The antibodies evenly distributed in the animals including the tumors. This antibody entered into both cytoplasm and nuclear mainly in the tumor cells (US20070243191). Immunohistochemistry with this antibody was performed on tissues from NSCLC patients (US20070243191). NSCLC tissue was injected in mice and later harvested. This harvested tumor tissue was then used for immunohistochemistry with this antibody (Zhao et al, 2013; pmid:23695169). Western blot was performed on HDGF protein using this antibody (Ren et al, 2009;pmid:19435872). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |