Mfg Part Number
Ab02461-3.0
Vector Labs Ab02461-3.0 Anti-EGFR [2224], Mouse IgG2b, lambda (200 μg)
Quick Overview
The original antibody was created using phage display from large naïve human scFv libraries.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 2224 |
| Synonyms: | ERBB1; ERBB; Erb-B2; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 |
| Amount: | 200 μg |
| Application Codes Clone: | FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for human EGFR. |
| Application Notes: | This antibody is derived after affinity maturation of a parental anti-EGFR clone C10. The original scFv antibody showed a 280 fold increase in affinity compared to the parental scFv clone C10. This antibody was used for analysis of scFv-mediated internalization of fluorescent immunoliposomes and quantification by microscopy and flow cytometry. This antibody bound to human EGFR expressed on MDAMB468 cells, A431 cells and EGFR (vIII) stably transfected NR6M and was detected by flow cytometry (Zhou et al, 2007; pmid:17602702). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |