Mfg Part Number
Ab02476-2.0
Vector Labs Ab02476-2.0 Anti-ICOS ligand [Clone 323], Mouse IgG2a, lambda (200 μg)
Quick Overview
The original antibody was isolated from a human naïve scFv phage library and panning against B7RP-1-Fc protein.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | Clone 323 |
| Synonyms: | CD275; ICOSLG; ICOSL; B7-H2; B7H2; B7RP-1; B7RP1; KIAA0653; B7 homolog 2; B7-like protein GL50; B7-related protein 1 |
| Amount: | 200 μg |
| Application Codes Clone: | Block; inhibit; FC; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds human B7RP-1, which is a ligand for T-cell-specific cell surface receptor ICOS. It acts as a costimulatory signal for T-cell proliferation and cytokine secretion and also induces B-cell proliferation and differentiation into plasma cells. |
| Application Notes: | The scFv version of this antibody binds B7RP-1-Fc with a Kd= 9nM. The binding specificity of this antibody was confirmed using ELISA. This scFv version of this antibody was shown to bind B7RP-1-Fc in an ELISA. This antibody recognized B7RP-1 molecules expressed on B cells, as assessed by flow cytometry. This antibody is capable of inhibiting interaction between ICOS-Fc and B7RP-1-Fc. This antibody also exhibited inhibitory activity in a proliferation assay of T cells stimulated with anti-CD3 mAb and B7RP-1-Fc (PMID: 20065650). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |