Vector Labs Ab02482-2.3 Anti-CD44 [F8:33], Mouse IgG2a, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: F8:33
Heavy Chain Modification: Fc Silent™
Synonyms: CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1
Amount: 200 μg
Application Codes Clone: ELISA; FC; therapeutic; functional assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for CD44vRA, which is a CD44 derived from rheumatoid arthritis patients. CD44vRA contains an extra alanine. At higher concentration it will also bind to other isoforms of CD44. CD44 is cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment.
Application Notes: To test the ability of the mouse version of this antibody to bind to CD44vRA, flow cytometry was preformed on Namalwa cells expressing different isoforms of CD44 (Golan et al, 2007; pmid:17383158). Further, it was used in ELISA preformed on different isotypes of the CD44 protein to assess the ability of this antibody to bind specific types of CD44; CD44vRA (CD44 from rheumatoid arthritis patients) was bound most strongly (Golan et al, 2007; pmid:17383158). Furthermore, flowcytometry was preformed to analyse apoptosis of synovial fluid cells. The mouse version of this antibody was used to induced apoptosis (Golan et al, 2007; pmid:17383158). It was administred to arthritic mice to reduce joint inflammation and induced apoptosis above the spontaneous level in the spleen of the mice (Golan et al, 2007; pmid:17383158). Transwell migration assay was preformed to assess the specificity of this antibody. It prevented namalwa-CD44vRA cells from crossing but did not prevent namalwa cells transfected with other forms of CD44 from crossing (US2011207678). Finally, flow cytometry was preformed with this antibody on synovial fluid cells and PBLs. The antibodies bound to the synovial fluid cells but not the PBLs from the same patient (Golan et al, 2007; pmid:17383158).

Product Class:	Purified
Clone ID:	F8:33
Heavy Chain Modification:	Fc Silent™
Synonyms:	CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1
Amount:	200 μg
Application Codes Clone:	ELISA; FC; therapeutic; functional assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for CD44vRA, which is a CD44 derived from rheumatoid arthritis patients. CD44vRA contains an extra alanine. At higher concentration it will also bind to other isoforms of CD44. CD44 is cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment.
Application Notes:	To test the ability of the mouse version of this antibody to bind to CD44vRA, flow cytometry was preformed on Namalwa cells expressing different isoforms of CD44 (Golan et al, 2007; pmid:17383158). Further, it was used in ELISA preformed on different isotypes of the CD44 protein to assess the ability of this antibody to bind specific types of CD44; CD44vRA (CD44 from rheumatoid arthritis patients) was bound most strongly (Golan et al, 2007; pmid:17383158). Furthermore, flowcytometry was preformed to analyse apoptosis of synovial fluid cells. The mouse version of this antibody was used to induced apoptosis (Golan et al, 2007; pmid:17383158). It was administred to arthritic mice to reduce joint inflammation and induced apoptosis above the spontaneous level in the spleen of the mice (Golan et al, 2007; pmid:17383158). Transwell migration assay was preformed to assess the specificity of this antibody. It prevented namalwa-CD44vRA cells from crossing but did not prevent namalwa cells transfected with other forms of CD44 from crossing (US2011207678). Finally, flow cytometry was preformed with this antibody on synovial fluid cells and PBLs. The antibodies bound to the synovial fluid cells but not the PBLs from the same patient (Golan et al, 2007; pmid:17383158).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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