Vector Labs Ab02486-1.1 Anti-MSCP [LC007], Mouse IgG1, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: LC007
Synonyms: Mitoferrin-1; SLC25A37; Mitochondrial iron transporter 1; Mitochondrial solute carrier protein; Solute carrier family 25 member 37
Amount: 200 μg
Application Codes Clone: FC; functional assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for repeat 14 of MSCP or for an epitope that is only reconstituted when repeat 14 is present. It also binds to cynomolgus MSCP. MSCP is a mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells, thereby playing an essential role in heme biosynthesis. The iron delivered into the mitochondria, presumably as Fe2+, is then probably delivered to ferrochelatase to catalyze Fe2+ incorporation into protoprophyrin IX to make heme (By similarity).
Application Notes: The binding epitope of this antibody was mapped by preforming flow cytometry on 4 different constructs. These were: construct 1 containing CSPG repeat 15, construct 2 containing CSPG repeat 14-15, construct 3 containing CSPG repeat 13-15, and construct 4 containing CSPG repeat 12-15 (US2014242083). Flow cytomtery was used to determine whether the human version of this antibody has cross-reactivity with human and cynomolgus antigen. The cells used were HEK-EBNA expressing either a construct containing the C-terminal part of the cynomolgus MCSP protein, a signal peptide for secretion and a N-terminal FLAG-tag or the human variant of this construct (US2014242083). Further, lysis of Colo38 human malignant melanoma cells by human lymphocytes was assessed using the glycoengineerd mouse version of this antibody (US2014242083). Co-culturing CD16-CAR T cells with A375 cells in presence of the human version of this antibody resulted in a dose-dependent increase in activation for the human version of this antibody (Rataj et al, 2018; pmid:30429531).

Product Class:	Purified
Clone ID:	LC007
Synonyms:	Mitoferrin-1; SLC25A37; Mitochondrial iron transporter 1; Mitochondrial solute carrier protein; Solute carrier family 25 member 37
Amount:	200 μg
Application Codes Clone:	FC; functional assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for repeat 14 of MSCP or for an epitope that is only reconstituted when repeat 14 is present. It also binds to cynomolgus MSCP. MSCP is a mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells, thereby playing an essential role in heme biosynthesis. The iron delivered into the mitochondria, presumably as Fe2+, is then probably delivered to ferrochelatase to catalyze Fe2+ incorporation into protoprophyrin IX to make heme (By similarity).
Application Notes:	The binding epitope of this antibody was mapped by preforming flow cytometry on 4 different constructs. These were: construct 1 containing CSPG repeat 15, construct 2 containing CSPG repeat 14-15, construct 3 containing CSPG repeat 13-15, and construct 4 containing CSPG repeat 12-15 (US2014242083). Flow cytomtery was used to determine whether the human version of this antibody has cross-reactivity with human and cynomolgus antigen. The cells used were HEK-EBNA expressing either a construct containing the C-terminal part of the cynomolgus MCSP protein, a signal peptide for secretion and a N-terminal FLAG-tag or the human variant of this construct (US2014242083). Further, lysis of Colo38 human malignant melanoma cells by human lymphocytes was assessed using the glycoengineerd mouse version of this antibody (US2014242083). Co-culturing CD16-CAR T cells with A375 cells in presence of the human version of this antibody resulted in a dose-dependent increase in activation for the human version of this antibody (Rataj et al, 2018; pmid:30429531).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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