Vector Labs Ab02489-10.3 Anti-IGF-IR [7C10], Human IgG1, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 7C10
Heavy Chain Modification: Fc Silent™
Synonyms: Insulin-like growth factor 1 receptor; IGF1R; Insulin-like growth factor I receptor; IGF-I receptor; CD221
Amount: 200 μg
Application Codes Clone: ELISA; WB; functional assay; therapeutic
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The antibody is specific for human IGF-IR. It does not prevent clone 12BI from binding to the same protein. IGF-IR is a receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control.
Application Notes: Specificity of this antibody was confirmed by ELISA (WO2009017679). Further, western blot was preformed to test whether the antibody cross reacts with IR protein (WO2009017679). It was also observed that the human version of this antibody was useful for antibody dependent cell cytotoxicity assays; MCF-7 or A549 target cells were incubated with this antibody and human natural killer cells. It was shown that the antibody does increase lysis of these cells (Goetsch et al, 2005; pmid:15386423). Furthermore, it was shown that the human version of this antibody is able to inhibit growth of MDA-MB-231, an oestrogen-independent breast cancer cell line in mice (Pandini et al, 2007; pmid:17451939). The human version of this antibody was also used in immunofluorescence on MCF-7 cells (Broussas et al, 2009; pmid:19165858). Finally, human version of this antibody was utilized in western blot of MCF-7 cells tumors (Broussas et al, 2009; pmid:19165858).

Product Class:	Purified
Clone ID:	7C10
Heavy Chain Modification:	Fc Silent™
Synonyms:	Insulin-like growth factor 1 receptor; IGF1R; Insulin-like growth factor I receptor; IGF-I receptor; CD221
Amount:	200 μg
Application Codes Clone:	ELISA; WB; functional assay; therapeutic
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The antibody is specific for human IGF-IR. It does not prevent clone 12BI from binding to the same protein. IGF-IR is a receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control.
Application Notes:	Specificity of this antibody was confirmed by ELISA (WO2009017679). Further, western blot was preformed to test whether the antibody cross reacts with IR protein (WO2009017679). It was also observed that the human version of this antibody was useful for antibody dependent cell cytotoxicity assays; MCF-7 or A549 target cells were incubated with this antibody and human natural killer cells. It was shown that the antibody does increase lysis of these cells (Goetsch et al, 2005; pmid:15386423). Furthermore, it was shown that the human version of this antibody is able to inhibit growth of MDA-MB-231, an oestrogen-independent breast cancer cell line in mice (Pandini et al, 2007; pmid:17451939). The human version of this antibody was also used in immunofluorescence on MCF-7 cells (Broussas et al, 2009; pmid:19165858). Finally, human version of this antibody was utilized in western blot of MCF-7 cells tumors (Broussas et al, 2009; pmid:19165858).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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