Vector Labs Ab02509-10.3 Anti-Influenza A virus NS1 [NS1-23-1], Human IgG1, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: NS1-23-1
Heavy Chain Modification: Fc Silent™
Synonyms: Non-structural protein 1; NS1; NS1A
Amount: 200 μg
Application Codes Clone: WB; IP
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for NS1 protein form influenza A. NS1 inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor/CPSF4 and the poly(A)-binding protein 2/PABPN1.
Application Notes: To research Akt phosphorylation, western blotting with this antibody was preformed on MDCK and Vero cells infected with A/Puerto Rico/8/34, B/Lee/40 or B/Maryland/59 (Ehrhardt et al, 2007; pmid:17715214). This antibody was used to asses the viral protein expression in A549, HEK293 and Vero cells via Western blot (Kathum et al, 2016; pmid:26687707). To elucidate the ability of NS1 to bind RNA, immunoprecipitation was preformed on cell lysates from A549 cells transfected with wt NS1 or mutated versions of NS1 (Kathum et al, 2016; pmid:26687707). For analysis of NS1-RIG-I complex formation, HEK293 cells were transfected with a pCAGGS-Flag-RIG-I construct. The cell lysate was then co-immunprecipitated using this antibody (Kathum et al, 2016; pmid:26687707). Further, the co-immunoprecipitated NS1 was detected by Western blot using this antibody (Kathum et al, 2016; pmid:26687707).

Product Class:	Purified
Clone ID:	NS1-23-1
Heavy Chain Modification:	Fc Silent™
Synonyms:	Non-structural protein 1; NS1; NS1A
Amount:	200 μg
Application Codes Clone:	WB; IP
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for NS1 protein form influenza A. NS1 inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor/CPSF4 and the poly(A)-binding protein 2/PABPN1.
Application Notes:	To research Akt phosphorylation, western blotting with this antibody was preformed on MDCK and Vero cells infected with A/Puerto Rico/8/34, B/Lee/40 or B/Maryland/59 (Ehrhardt et al, 2007; pmid:17715214). This antibody was used to asses the viral protein expression in A549, HEK293 and Vero cells via Western blot (Kathum et al, 2016; pmid:26687707). To elucidate the ability of NS1 to bind RNA, immunoprecipitation was preformed on cell lysates from A549 cells transfected with wt NS1 or mutated versions of NS1 (Kathum et al, 2016; pmid:26687707). For analysis of NS1-RIG-I complex formation, HEK293 cells were transfected with a pCAGGS-Flag-RIG-I construct. The cell lysate was then co-immunprecipitated using this antibody (Kathum et al, 2016; pmid:26687707). Further, the co-immunoprecipitated NS1 was detected by Western blot using this antibody (Kathum et al, 2016; pmid:26687707).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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