Vector Labs Ab02510-10.3 Anti-Talin 1 [8D4], Human IgG1, Fc Silent™, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 8D4
Heavy Chain Modification: Fc Silent™
Synonyms: talin 1; Talin1; talin-1; TLN; TLN1
Amount: 100 μg
Application Codes Clone: WB; ELISA; FC; IP; ICC; IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The antibody is specific for mammalian and avian talin. Talin is a multifunctional constituent of cell-substratum attachment sites and it has a high molecular weight (225-235 kDa). The protein is found in a variety of tissues and cell types.
Application Notes: The antibody detected the N-terminal region of talin1 by western blot analysis (Praekelt et al., 2012; PMID: 22306379 and Martel et al., 2001; PMID: 11279249). However, it also detected the equivalent region of talin2, albeit to a lesser degree (Praekelt et al., 2012; PMID: 22306379). This antibody was used for detection of talin expression on HeLa/AT22, AT21, wild-type HeLa cells or pMEP4-transfected HeLa cells cells by flow cytometry (Albigès-Rizo et al., 1995; PMID: 7593292). lmmunocytochemistry using the antibody showed patterns of talin expression within DRG growth cones (Sydor et al., 1996; PMID: 8794861). Indirect immunofluorescence staining of cultured fibroblasts (rat, human, chicken and xenopus embryo fibroblasts) was tested with the antibody. In all the samples the focal adhesions were stained with the antibody (Otey et al. 1990; PMID: 1690175). Talin was immunoprecipitated from MDA-MB-231 cell lysates using the antibody (Beaty et al., 2014; PMID: 24891603).

Product Class:	Purified
Clone ID:	8D4
Heavy Chain Modification:	Fc Silent™
Synonyms:	talin 1; Talin1; talin-1; TLN; TLN1
Amount:	100 μg
Application Codes Clone:	WB; ELISA; FC; IP; ICC; IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The antibody is specific for mammalian and avian talin. Talin is a multifunctional constituent of cell-substratum attachment sites and it has a high molecular weight (225-235 kDa). The protein is found in a variety of tissues and cell types.
Application Notes:	The antibody detected the N-terminal region of talin1 by western blot analysis (Praekelt et al., 2012; PMID: 22306379 and Martel et al., 2001; PMID: 11279249). However, it also detected the equivalent region of talin2, albeit to a lesser degree (Praekelt et al., 2012; PMID: 22306379). This antibody was used for detection of talin expression on HeLa/AT22, AT21, wild-type HeLa cells or pMEP4-transfected HeLa cells cells by flow cytometry (Albigès-Rizo et al., 1995; PMID: 7593292). lmmunocytochemistry using the antibody showed patterns of talin expression within DRG growth cones (Sydor et al., 1996; PMID: 8794861). Indirect immunofluorescence staining of cultured fibroblasts (rat, human, chicken and xenopus embryo fibroblasts) was tested with the antibody. In all the samples the focal adhesions were stained with the antibody (Otey et al. 1990; PMID: 1690175). Talin was immunoprecipitated from MDA-MB-231 cell lysates using the antibody (Beaty et al., 2014; PMID: 24891603).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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