JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.

Skip to the beginning of the images gallery

Mfg Part Number

Ab02511-3.3

Vector Labs Ab02511-3.3 Anti-Thomsen-nouveaupolysaccharide A1 [KT-IgM-8], Mouse IgG2b, Fc Silent™, kappa (100 μg)

VECTOR LABS

Be the first to review this product

Quick Overview

The original antibody was generated by immunizing C57BL/6 mice against Tn-PS A1.

$570.70

Order will ship within 1-3 business days

Description

Product Class: Purified
Clone ID: KT-IgM-8
Heavy Chain Modification: Fc Silent™
Synonyms: Tn-PS A1; Thomsen-nouveau (Tn); Tn antigen; Tn saccharide; N-acetyl Galactosamine; Alpha-GalNAc; Alpha GalpNAc; GalNAc; GalNAc-alpha; N-acetyl-alpha-D-galactosamine; Blood group A antigen
Amount: 100 μg
Application Codes Clone: ELISA; FC; Complement‑dependent cytotoxicity; glycan array technologies; in vivo; in vitro
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgM
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The antibody is specific for Tn-PS A1. It does not cross react with PS A1.
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The ability of the antibody to bind cancer cells expressing the Tn antigen on the cell surface (MCF-7 and HCT-116) was evaluated by by FC. The antibody demonstrated the ability to bind both MCF-7 and HCT-116 tumor cells at 30 µg/mL with a shift in fluorescence of 49% in both cell lines. The antibody demonstrated in vitro tumor killing using an LDH assay. The assay measured that the antibody-induced complement-dependent cytotoxicity was 30%. In vivo passive immunotherapy approach using an MCF-7 cell line-derived xenograft model was evaluated. The antibody demonstrated approximately 40% reduction in tumor growth in the MCF-7 model (Trabbic et al., 2018; PMID: 30030557).

Product Class:	Purified
Clone ID:	KT-IgM-8
Heavy Chain Modification:	Fc Silent™
Synonyms:	Tn-PS A1; Thomsen-nouveau (Tn); Tn antigen; Tn saccharide; N-acetyl Galactosamine; Alpha-GalNAc; Alpha GalpNAc; GalNAc; GalNAc-alpha; N-acetyl-alpha-D-galactosamine; Blood group A antigen
Amount:	100 μg
Application Codes Clone:	ELISA; FC; Complement‑dependent cytotoxicity; glycan array technologies; in vivo; in vitro
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgM
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The antibody is specific for Tn-PS A1. It does not cross react with PS A1.
Application Notes:	The specificity of the original format of the antibody was confirmed by ELISA analysis. The ability of the antibody to bind cancer cells expressing the Tn antigen on the cell surface (MCF-7 and HCT-116) was evaluated by by FC. The antibody demonstrated the ability to bind both MCF-7 and HCT-116 tumor cells at 30 µg/mL with a shift in fluorescence of 49% in both cell lines. The antibody demonstrated in vitro tumor killing using an LDH assay. The assay measured that the antibody-induced complement-dependent cytotoxicity was 30%. In vivo passive immunotherapy approach using an MCF-7 cell line-derived xenograft model was evaluated. The antibody demonstrated approximately 40% reduction in tumor growth in the MCF-7 model (Trabbic et al., 2018; PMID: 30030557).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews