Mfg Part Number
Ab02512-10.3
Vector Labs Ab02512-10.3 Anti-properdin [3A3E1], Human IgG1, Fc Silent™, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing mice against purified human properdin.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | 3A3E1 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | factor P; Complement factor P; properdin mab |
| Amount: | 100 μg |
| Application Codes Clone: | WB; ELISA; AP-Mediated Hemolytic Assays |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The antibody is specific for properdin. It recognizes human, baboon, rabbit, and canine properdin, but not mouse, rat, feline or ferret properdin. |
| Application Notes: | The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected only non-reduced properdin as evaluated by western blot analysis. The antibody inhibited AP-mediated hemolysis of rabbit erythrocytes. The ability of the antibody to inhibit the binding of properdin to C3b was tested by flow cytometry. The antibody completely impaired the binding of properdin to C3b-coated erythrocytes (ESC3b) (Chen et al. 2020; PMID: 32793201). Further, the antibody inhibited PGA formation relative to TRAP alone (Blatt et al., 2016; PMID: 27183616). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |