Vector Labs Ab02531-10.3 Anti-FGRI [M19B2], Human IgG1, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: M19B2
Heavy Chain Modification: Fc Silent™
Synonyms: Fibroblast growth factor receptor 1; FGFR-1; Basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; Fms-like tyrosine kinase 2; FLT-2; N-sam; Proto-oncogene c-Fgr; CD331
Amount: 200 μg
Application Codes Clone: WB; IP; FC; IHC; IF; RIA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for the acidic box and heparin-binding domain. FGFR is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.
Application Notes: To characterize this antibody a western blot was preformed on sf9 cells transfected with baculovirus recombinantly expressing the full length FGF-R1 protein. Furthermore immunoprecipitation was preformed on the lysed sf9 cells transfected with baculovirus recombinantly expressing the full length FGF-R1 protein. And finally a radioimmunityassay was preformed with the mouse version of this antibody on I125-labeled complexes (Xu et al, 1992; pmid:1325449). During research on the localization and behaviour of the FGF-1 protein the murine version of this antibody was used to immunoprecipitate FGFR and a 40-kDa complex it is part of (Feng et al, 1995; pmid:9244177). To confirm that Klotho-expressing B cells could respond to FGF23, their expression of FGFR1 was assayed by flow cytometry and immunohistochemical staining. For this the mouse version of this antibody was used (Nakashima et al, 2016; pmid:28095739). fluorescence immunocytochemistry was preformed on brain slices or brain cells from FGF-2 mice and FGF-2 knock-out mice using the mouse version of this antibody. This was done to prove that nuclear FGFR1 and Nurr1 are expressed in the nuclei of developing TH-positive cells in the embryonic ventral midbrain (Baron et al, 2012; pmid:22514272). While investigating a method to establish functional and stable neovessel networks to increase plaque stability The murine version of this antibody was used for western blot on proteins extracted from rabbit aortas and cell lysates (Mao et al, 2019; pmid:31755222).

Product Class:	Purified
Clone ID:	M19B2
Heavy Chain Modification:	Fc Silent™
Synonyms:	Fibroblast growth factor receptor 1; FGFR-1; Basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; Fms-like tyrosine kinase 2; FLT-2; N-sam; Proto-oncogene c-Fgr; CD331
Amount:	200 μg
Application Codes Clone:	WB; IP; FC; IHC; IF; RIA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for the acidic box and heparin-binding domain. FGFR is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.
Application Notes:	To characterize this antibody a western blot was preformed on sf9 cells transfected with baculovirus recombinantly expressing the full length FGF-R1 protein. Furthermore immunoprecipitation was preformed on the lysed sf9 cells transfected with baculovirus recombinantly expressing the full length FGF-R1 protein. And finally a radioimmunityassay was preformed with the mouse version of this antibody on I125-labeled complexes (Xu et al, 1992; pmid:1325449). During research on the localization and behaviour of the FGF-1 protein the murine version of this antibody was used to immunoprecipitate FGFR and a 40-kDa complex it is part of (Feng et al, 1995; pmid:9244177). To confirm that Klotho-expressing B cells could respond to FGF23, their expression of FGFR1 was assayed by flow cytometry and immunohistochemical staining. For this the mouse version of this antibody was used (Nakashima et al, 2016; pmid:28095739). fluorescence immunocytochemistry was preformed on brain slices or brain cells from FGF-2 mice and FGF-2 knock-out mice using the mouse version of this antibody. This was done to prove that nuclear FGFR1 and Nurr1 are expressed in the nuclei of developing TH-positive cells in the embryonic ventral midbrain (Baron et al, 2012; pmid:22514272). While investigating a method to establish functional and stable neovessel networks to increase plaque stability The murine version of this antibody was used for western blot on proteins extracted from rabbit aortas and cell lysates (Mao et al, 2019; pmid:31755222).

Specifications

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Brand	Vector Labs
UNSPSC	12161500

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