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Mfg Part Number

Ab02543-9.4

Vector Labs Ab02543-9.4 Anti-(1-4)-β-D-galactan [LM5], Rat IgG2c, Fc Silent™, kappa (200 μg)

VECTOR LABS

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Quick Overview

The rat version of this antibody was raised by immunizing a wister rat with a polysaccharide.

$517.40

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Description

Product Class: Purified
Clone ID: LM5
Heavy Chain Modification: Fc Silent™
Amount: 200 μg
Application Codes Clone: ELISA; WB; functional assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2c
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for beta-1,4-galactan. It specifically recognized more than 3 contiguous units of beta-1,4-galactan. Beta-1,4-galactan is expressed prefominantly in the cell wall.
Application Notes: To characterize this antibody, a competitive ELISA was performed on saccharides using the original rat version of this antibody. Furthermore, an immunoblot was performed on citrus pectin, apple pectin, TG1, TG2a, TG2b, GALCBSA, BSA, lupin galactan, gum arabic, polygalacturonic acid, larchwood arabinogalactan, and locust bean gum using this antibody. Finally, tomato pericarp was immunogold labelled using this antibody (Jones et al, 1997; pmid:12223681). To map the epitope of this antibody, an ELISA was performed on different lengths of beta-1,4-galactan and beta-1,4-galactan with different side groups. It showed the greatest binding efficiency to 4 units of beta-1,4-galactan (Andersen et al, 2016; pmid:27855335). While creating and characterizing an antibody against (1→5)-α-l-arabinan, the rat version of this antibody was used as a control in an ELISA. Furthermore, a immunoblot was performed on surfaces of lemons using this antibody (Willats et al, 1998, pmid:9675359). Pea cotyledons were stained for immunofluorescence using the rat version of this antibody to study plant cell wall architecture. Furthermore, immunogold assay was performed on pea cotyledons using this antibody (McCartney et al, 2000; pmid:10792826).

Product Class:	Purified
Clone ID:	LM5
Heavy Chain Modification:	Fc Silent™
Amount:	200 μg
Application Codes Clone:	ELISA; WB; functional assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2c
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for beta-1,4-galactan. It specifically recognized more than 3 contiguous units of beta-1,4-galactan. Beta-1,4-galactan is expressed prefominantly in the cell wall.
Application Notes:	To characterize this antibody, a competitive ELISA was performed on saccharides using the original rat version of this antibody. Furthermore, an immunoblot was performed on citrus pectin, apple pectin, TG1, TG2a, TG2b, GALCBSA, BSA, lupin galactan, gum arabic, polygalacturonic acid, larchwood arabinogalactan, and locust bean gum using this antibody. Finally, tomato pericarp was immunogold labelled using this antibody (Jones et al, 1997; pmid:12223681). To map the epitope of this antibody, an ELISA was performed on different lengths of beta-1,4-galactan and beta-1,4-galactan with different side groups. It showed the greatest binding efficiency to 4 units of beta-1,4-galactan (Andersen et al, 2016; pmid:27855335). While creating and characterizing an antibody against (1→5)-α-l-arabinan, the rat version of this antibody was used as a control in an ELISA. Furthermore, a immunoblot was performed on surfaces of lemons using this antibody (Willats et al, 1998, pmid:9675359). Pea cotyledons were stained for immunofluorescence using the rat version of this antibody to study plant cell wall architecture. Furthermore, immunogold assay was performed on pea cotyledons using this antibody (McCartney et al, 2000; pmid:10792826).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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