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Mfg Part Number

Ab02544-23.0

Vector Labs Ab02544-23.0 Anti-Branched galactan [LM26], Rabbit IgG, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing rats with lime pectin.

$570.70

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Description

Product Class: Purified
Clone ID: LM26
Synonyms: D-Galactan; Galactosan; β-1,6-galactosyl substitution of β-1,4-galactan
Amount: 100 μg
Application Codes Clone: ELISA; IHC; IF; IEM; TEM; Immunogold labeling
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for the β-1,6-galactosyl substitution of β-1,4-galactan, a branched galactan epitope associated with pectic rhamnogalacturonan-I located in the inner cell wall regions adjacent to plasma membranes, specific to phloem sieve elements. The antibody requires more than three backbone residues for optimized recognition.
Application Notes: The binding specificity of this antibody was evaluated via carbohydrate (glycan) microarray screening and ELISA. This antibody was used for indirect fluorescence immunolabeling on various plant organs, including Arabidopsis inflorescence stems, tomato leaf petiole, Miscanthus x giganteus stem, and sugar beet storage roots. Additionally, it was used for immunoelectron microscopy (transmission electron microscopy and immunogold labeling) on transverse sections of Miscanthus stem and garlic bulb cell walls (Torode et al., 2018; PMID: 29150558). This antibody was used for immunohistochemical analysis of cell wall polymers in dissected maize roots (Kozlova et al., 2020; PMID: 32616810).

Product Class:	Purified
Clone ID:	LM26
Synonyms:	D-Galactan; Galactosan; β-1,6-galactosyl substitution of β-1,4-galactan
Amount:	100 μg
Application Codes Clone:	ELISA; IHC; IF; IEM; TEM; Immunogold labeling
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for the β-1,6-galactosyl substitution of β-1,4-galactan, a branched galactan epitope associated with pectic rhamnogalacturonan-I located in the inner cell wall regions adjacent to plasma membranes, specific to phloem sieve elements. The antibody requires more than three backbone residues for optimized recognition.
Application Notes:	The binding specificity of this antibody was evaluated via carbohydrate (glycan) microarray screening and ELISA. This antibody was used for indirect fluorescence immunolabeling on various plant organs, including Arabidopsis inflorescence stems, tomato leaf petiole, Miscanthus x giganteus stem, and sugar beet storage roots. Additionally, it was used for immunoelectron microscopy (transmission electron microscopy and immunogold labeling) on transverse sections of Miscanthus stem and garlic bulb cell walls (Torode et al., 2018; PMID: 29150558). This antibody was used for immunohistochemical analysis of cell wall polymers in dissected maize roots (Kozlova et al., 2020; PMID: 32616810).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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