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Mfg Part Number

Ab02550-10.3

Vector Labs Ab02550-10.3 Anti-Alpha-ketoglutarate-dependent dioxygenase FTO [5-2H10], Human IgG1, Fc Silent™, kappa (200 μg)

VECTOR LABS

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Quick Overview

This antigen was raised by immunizing a BALB/c mouse with the alpha-ketoglutarate-dependent dioxygenase FTO antigen.

$517.40

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Description

Product Class: Purified
Clone ID: 5-2H10
Heavy Chain Modification: Fc Silent™
Synonyms: FTO; Fat mass and obesity-associated protein; U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO; mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; m6A(m)-demethylase FTO; mRNA N(6)-methyladenosine demethylase FTO; tRNA N1-methyl adenine demethylase FTO
Amount: 200 μg
Application Codes Clone: WB; ICC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for the alpha-ketoglutarate-dependent dioxygenase FTO. This protein is a RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis. Specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes.
Application Notes: This antibody was used to assess the expression of the FTO protein in cell line 3T3-L1. The method used was a western blot (Jiao et al, 2016; pmid:26907332). Immunocytochemistry with this antibody was used for examination of FTO expression in HOC313, MDA-MB-231, SAS, HeLa, 293FT, and MKN45 cells. The experiment was preformed on normal cells and FTO knock-out cells (Hirayama et al, 2020; pmid:32268083).

Product Class:	Purified
Clone ID:	5-2H10
Heavy Chain Modification:	Fc Silent™
Synonyms:	FTO; Fat mass and obesity-associated protein; U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO; mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; m6A(m)-demethylase FTO; mRNA N(6)-methyladenosine demethylase FTO; tRNA N1-methyl adenine demethylase FTO
Amount:	200 μg
Application Codes Clone:	WB; ICC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for the alpha-ketoglutarate-dependent dioxygenase FTO. This protein is a RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis. Specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes.
Application Notes:	This antibody was used to assess the expression of the FTO protein in cell line 3T3-L1. The method used was a western blot (Jiao et al, 2016; pmid:26907332). Immunocytochemistry with this antibody was used for examination of FTO expression in HOC313, MDA-MB-231, SAS, HeLa, 293FT, and MKN45 cells. The experiment was preformed on normal cells and FTO knock-out cells (Hirayama et al, 2020; pmid:32268083).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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