Mfg Part Number
Ab02554-8.4
Vector Labs Ab02554-8.4 Anti-CD8 (Lyt-2.2) [2.43], Rat IgG2b, Fc Silent™, kappa (200 μg)
Quick Overview
This antibody was generated by immunizing a Lewis rat with i.v. 3 times at 2-wk intervals with 15 * 10^6 L3 cloned cytolytic T cells. Three days after the third immunization, the rat spleen cells were fused with either P3 or SP2/0 cells using polyethylene glycol.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 2.43 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Lyt 2.2; T-cell surface glycoprotein CD8 alpha chain; Cd8a; T-cell surface glycoprotein Lyt-2; lyt-2; lyt2; CD_antigen: CD8a; 2.43.1 |
| Amount: | 200 μg |
| Application Codes Clone: | IF; FC; blocking; IP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for the lyt-2 protein of mice (it is psecific to the Lyt2.2 variant which is expressed in the mouse strains BALB/c and C57BL/6 (B/6)). Lyt-2 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. |
| Application Notes: | This antibody was used for an immunofluorescence experiment on mouse thymocytes and mouse spleen cells (Sarmiento et al, 1980; pmid:6159417). Immunoprecipitation was preformed on NP-40 extract of 126I surfae labeled L3 cells using this antibody (Sarmiento et al, 1980; pmid:6159417). This antibody was used to try and block the cytolytic activity from L3 against P-185. It only blocks the receptor-mediated but not lectin mediated cytolysis by L3 (Dialynas et al, 1981; pmid:6788878). Flow cytometry was preformed with this antibody on washed cells from the lymph node, spleen, thymus and peripheral blood from mice (Rodriguez and Sriram, 1988; pmid:2966203). This antibody was used to stain mouse spleen cells for use in flow cytometry (Mielke et al, 1988; pmid:2969373). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |