Mfg Part Number
Ab02558-3.3
Vector Labs Ab02558-3.3 Anti-class I MHC [5H7], Mouse IgG2b, Fc Silent™, kappa (200 μg)
Quick Overview
BALB/c mice were immunized by a series of four to six monthly injections of human PBLs, human Jurkat tumor ceIls, or enriched T lymphocytes.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 5H7 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | HLA class I histocompatibility antigen; HLA; Human leukocyte antigen |
| Amount: | 200 μg |
| Application Codes Clone: | blocking; IP; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for HLA class I histocompatibility antigen all different chains. Antigen-presenting major histocompatibility complex class I (MHCI) molecule. In complex with B2M/beta 2 microglobulin displays primarily viral and tumor-derived peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-A-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected or transformed cells. |
| Application Notes: | The biotinylated antibody was used for a blocking experiment in human PBLs (Smith et al, 1994; pmid:7913109). Flow cytometry was preformed on human PBMC using this antibody (Woodle et al, 1997; pmid:9036961). This antibody was used for immunoprecipitation on C1R and T2. HMy2.C1R (C1R) are HLA-A negative and -B35 low human B-lymphoblastoid cells, and 0.174 X CEMR (T2) are T-B lymphoblastoid hybrid cells that are missing a large region of the MHC, including the genes encoding TAP1 and TAP2 (Dangoria et al, 2002; pmid:11978783). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |