Mfg Part Number
Ab02559-22.0
Vector Labs Ab02559-22.0 Anti-CTLA4 [1B8], Hamster (Armenian) IgG, kappa (200 μg)
Quick Overview
The antibody was generated by immunizing Armenian hamsters with the extracellular portion of murine CTLA-4 fused to a murine IgG2.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 1B8 |
| Synonyms: | Cytotoxic T-lymphocyte protein 4; Ctla4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CD152; UC10-4FIO |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC; IF; WB; blocking |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for CTLA-4. CTLA-4 is an inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. |
| Application Notes: | ELISA was preformed on murine CTLA-4lg to test the binding specificity of the anitbody (Walunas et al, 1994; pmid:7882171). For flow cytometry on A20, M12.41 (B cells), L cell (fibroblast), and P815 (mastocytoma) cells this antibody was used (Perkins et al, 1996; pmid:8666782). This antibody was used for blocking in mice. It prevented T-cell proliferation (Leach et al, 1996; pmid:8596936). Western blot was preformed on LN cells harvested from SJL mice using this antibody (Aruna et al, 2005; pmid:16014414). This antibody was used for flow cytometry on LN cells harvested from SJL mice (Aruna et al, 2005; pmid:16014414). Mouse CD4+ CD25+ T cells were used for a flow cytomic experiment using this antibody (Valsazina et al, 2006; pmid:16618776). Thymi from Tg4 CTLA-4WT and B10.PL |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |