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Mfg Part Number

Ab02560-1.1

Vector Labs Ab02560-1.1 Anti-Ly-6C (T cell activating) [HK1.4], Mouse IgG1, kappa (200 μg)

VECTOR LABS

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Quick Overview

The antibody was raised by immunizing a Lewis rat by a series of four monthly i.p. injections of approximately 30 X 10" L3 cloned CTL cells.

$517.40

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Description

Product Class: Purified
Clone ID: HK1.4
Synonyms: Lymphocyte antigen 6C1; Ly6c1
Amount: 200 μg
Application Codes Clone: FC; functional assay; IF; IP
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2c
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2c format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for Ly6 and shows no cross reactivity. Ly6 shows acetylcholine receptor binding activity.
Application Notes: A flow cytometry experiment on mouse cells from L-gD-1, an L cell line that expresses HSV type 1 gD, and L-gI-1, an L cell line that expresses HSV type 1 gI was preformed. In this experiment this antibody was used for reverse blocking (Johnson et al, 1993; pmid:8104217). Th1 clones from either wild-type or fyn-/- mice were used in an experiment where it was determined if this antibody could induce lysis (Lancki et al, 1995; pmid:7493751). Flow cytometry was preformed on cells from C57BL/6, DBAI2, NJ. BALB/c, PIJ, PL/J. AKRIJ. RIII, SM/J, SJL, and CBA mice using this antibody (Havran et al, 1988; pmid:3257767). Immunofluorescence was preformed on rat EL4J cells using this antibody (Fleming et al, 1993; pmid:8360469). EL4J and EUJ-Ly-6G cell lines were immunoprecipitated with this antibody (Fleming et al, 1993; pmid:8360469). Neonatal murine calvaria-derived osteoblasts were stained for flow cytometry using this antibody (Horowitz et al, 1994; pmid:7520861).

Product Class:	Purified
Clone ID:	HK1.4
Synonyms:	Lymphocyte antigen 6C1; Ly6c1
Amount:	200 μg
Application Codes Clone:	FC; functional assay; IF; IP
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2c
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2c format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for Ly6 and shows no cross reactivity. Ly6 shows acetylcholine receptor binding activity.
Application Notes:	A flow cytometry experiment on mouse cells from L-gD-1, an L cell line that expresses HSV type 1 gD, and L-gI-1, an L cell line that expresses HSV type 1 gI was preformed. In this experiment this antibody was used for reverse blocking (Johnson et al, 1993; pmid:8104217). Th1 clones from either wild-type or fyn-/- mice were used in an experiment where it was determined if this antibody could induce lysis (Lancki et al, 1995; pmid:7493751). Flow cytometry was preformed on cells from C57BL/6, DBAI2, NJ. BALB/c, PIJ, PL/J. AKRIJ. RIII, SM/J, SJL, and CBA mice using this antibody (Havran et al, 1988; pmid:3257767). Immunofluorescence was preformed on rat EL4J cells using this antibody (Fleming et al, 1993; pmid:8360469). EL4J and EUJ-Ly-6G cell lines were immunoprecipitated with this antibody (Fleming et al, 1993; pmid:8360469). Neonatal murine calvaria-derived osteoblasts were stained for flow cytometry using this antibody (Horowitz et al, 1994; pmid:7520861).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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