Vector Labs Ab02567-6.4 Anti-estrogen receptor alpha [H222.2], Rat IgG1, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: H222.2
Heavy Chain Modification: Fc Silent™
Synonyms: Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR1
Amount: 200 μg
Application Codes Clone: ELISA; IP; IHC; WB; functional assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for human estrogen receptor. The estrogen receptor is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling.
Application Notes: ELISA was preformed on human breast cancer tissue, human,monkey and calf uterus tissue and hen oviduct tissue. For this ELISA this antibody was used (Greene et al, 1984; pmid:6200700). Semi-confluent HeLa cells (3-5 x 106 cells/9 cm Petri dish) transfected with 10 ug of recombinant DNA (HEO-HE14) were immunoprecipitated with this antibody (Kumar et al, 1986; pmid:3780678). Immunohistochemistry was preformed, to compare them to their own antibodies, on human breast cancers using this antibody (Saati et al , 1993; pmid:8406995). Western blot was preformed on protein extracts from (i) transformed E. coli; (ii) COS cells transfected with pSG5-HGO or pSG5-HG19 (containing a deletion mutant of the ER cDNA lacking the sequence coding for the 178 N terminal amino-acids) the human breast-cancer cell line MCF-7 (ATCC HTB 22, Rockville, MD) and (iv) endometrial tissue. In this western blotting this antibody was used (Saati et al , 1993; pmid:8406995). This antibody was used for immunohistochemistry on brain tissue of female rats (Henry Jr et al, 1991; pmid:1917572). Immunohistochemistry was preformed on tissue samples from surgically removed human uteri using this antibody (Charpin et al, 1986; pmid:3522449). Sucrose density gradients were preformed on anterior oviduct or liver tissue from a red-sided garter snake using this antibody (Whittier et al, 1991; pmid:2026308). A steroid specificty trail was preformed on anterior oviduct or liver tissue from a red-sided garter snake using this antibody (Whittier et al, 1991; pmid:2026308). to characterize the putative membrane ER on human spermatozoa, western blot was preformed on human sperm lysates (Luconi et al, 2004; pmid:15288769).

Product Class:	Purified
Clone ID:	H222.2
Heavy Chain Modification:	Fc Silent™
Synonyms:	Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR1
Amount:	200 μg
Application Codes Clone:	ELISA; IP; IHC; WB; functional assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for human estrogen receptor. The estrogen receptor is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling.
Application Notes:	ELISA was preformed on human breast cancer tissue, human,monkey and calf uterus tissue and hen oviduct tissue. For this ELISA this antibody was used (Greene et al, 1984; pmid:6200700). Semi-confluent HeLa cells (3-5 x 106 cells/9 cm Petri dish) transfected with 10 ug of recombinant DNA (HEO-HE14) were immunoprecipitated with this antibody (Kumar et al, 1986; pmid:3780678). Immunohistochemistry was preformed, to compare them to their own antibodies, on human breast cancers using this antibody (Saati et al , 1993; pmid:8406995). Western blot was preformed on protein extracts from (i) transformed E. coli; (ii) COS cells transfected with pSG5-HGO or pSG5-HG19 (containing a deletion mutant of the ER cDNA lacking the sequence coding for the 178 N terminal amino-acids) the human breast-cancer cell line MCF-7 (ATCC HTB 22, Rockville, MD) and (iv) endometrial tissue. In this western blotting this antibody was used (Saati et al , 1993; pmid:8406995). This antibody was used for immunohistochemistry on brain tissue of female rats (Henry Jr et al, 1991; pmid:1917572). Immunohistochemistry was preformed on tissue samples from surgically removed human uteri using this antibody (Charpin et al, 1986; pmid:3522449). Sucrose density gradients were preformed on anterior oviduct or liver tissue from a red-sided garter snake using this antibody (Whittier et al, 1991; pmid:2026308). A steroid specificty trail was preformed on anterior oviduct or liver tissue from a red-sided garter snake using this antibody (Whittier et al, 1991; pmid:2026308). to characterize the putative membrane ER on human spermatozoa, western blot was preformed on human sperm lysates (Luconi et al, 2004; pmid:15288769).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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