Mfg Part Number
Ab02573-10.3
Vector Labs Ab02573-10.3 Anti-IgD (Igh-Ca haplotype) [AMS9- 9.1.1.1], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
This antibody was raised by immunizing C57BL/6 mice with spleen cells.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | AMS9- 9.1.1.1 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | IgD |
| Amount: | 200 μg |
| Application Codes Clone: | FC; IF; IHC; functional assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for IgD (Igh-Ca haplotype). |
| Application Notes: | PI- cells were used in an flow cytometry experiment using this antibody (Stall and Loken, 1984; pmid:6690619). A competitive binding assay on BALB/c mouse spleen cells was preformed using a radiolabelled version of this antibody (May et al, 1990; pmid:1691764). A cytotoxicity assay was preformed on mouse lymph node cells using this antibody. The antibody was 62,5 fold less toxic than ricin (May et al, 1990; pmid:1691764). BALB/c mice were given this antibody as a preventive treatment for chronic TMA- and DNCB-induced CHS (Nguyen, 2018; pmid:30218740). ELISA was preformed on TMA-specific IgM, IgG and IgE in mouse serum with this antibody (Nguyen, 2018; pmid:30218740). Fresh mouse spleen cells were used in flow cytometry using this antibody (Nguyen, 2018; pmid:30218740). B cells from mice were used for immunofluorescence with this antibody (Pereira et al, 2009; pmid:19597478). Horizontal 5 um sections of mice were used for immunohistochemistry using this antibody (Zuercher et al, 2002; pmid:11823512). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |