Mfg Part Number
Ab02575-4.3

Vector Labs Ab02575-4.3 Anti-a3 domain of H-2Kd MHC class I [SF1-1.1.1], Mouse IgG2c, Fc Silent™, kappa (200 μg)

Quick Overview
this antibody was raised against the mouse a3 domain of H-2Kd MHC class I antigen.
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Product Class:Purified
Clone ID:SF1-1.1.1
Heavy Chain Modification:Fc Silent™
Synonyms:H-2 class I histocompatibility antigen, K-D alpha chain; H2-K1; H-2K(D)
Amount:200 μg
Application Codes Clone:WB; FC; IHC; IP; IF
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG2c
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:This antibody is specific for a3 domain of mouse H-2Kd MHC class I. Involved in the presentation of foreign antigens to the immune system.
Application Notes:A dot blot was preformed with this antibody on strain MW98-8C of the yeast Kluyveromyces luctis with single Ala substitutions of the native Kd molecule (Abastado et al, 1993; pmid:8376793). This antibody was used for flow cytometry on spleen cells from a Balb/c (H-2Kd) mouse (Bjornson et al, 1999; pmid:9915700). H-2Kd molecules were immunoaffinity-purified from 1.4 × 1010 IFN-γ-treated NIT-1 pancreatic β cells using this antibody (Lieberman et al, 2003; pmid:12815107). RMA-S/Kd cells were immunostained with this antibody (Lieberman et al, 2003; pmid:12815107). grafted organs and spleens from C57BL/6 mice were used for immunohistochemistry using this antibody (Brown et al, 2011; pmid:21401861).
More Information
Brand Vector Labs
UNSPSC 12161500