Mfg Part Number
Ab02589-23.0
Vector Labs Ab02589-23.0 Anti-IL4 [6D9G1], Rabbit IgG, kappa (200 μg)
Quick Overview
The original antibody was raised by immunising BALB/C mice with recombinant chicken IL-4.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | 6D9G1 |
| Synonyms: | Interleukin 4; IL-4; chIL-4; chicken IL-4; B-cell stimulatory factor 1; Lymphocyte stimulatory factor 1 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; ICC; FC; neutralizing |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds specifically to chicken IL-4, with no cross reactivities with human or mouse IL-4 and other chicken cytokines. |
| Application Notes: | The interleukin 4 is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. The antibody was used to explore the functionality of chicken IL-4. The specificity of this antibody (IgG2a) was confirmed in ELISA analysis (Chaudhari et al, PMID: 30459004). The antibody detected the recombinant chIL-4 by western blot. Similarly, the antibody detected chIL-4 production in HD11 cells by immunocytochemistry. This antibody was also used in flow cytometry analysis. The antibody was used in in vitro neutralization assay using HD11 cells and primary monocytes (Chaudhari et al, 2018; pmid: 30459004). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |