Mfg Part Number
Ab02591-23.0

Vector Labs Ab02591-23.0 Anti-IL-1B [1], Rabbit IgG, kappa (200 μg)

Quick Overview
The original antibody was raised immunising BALB/C mice with recombinant chicken IL-1B protein.
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Product Class:Purified
Clone ID:1
Synonyms:Interleukin IL1‐beta; chIL-1B-1; Interleukin-1; chIL-1B
Amount:200 μg
Application Codes Clone:ELISA; WB; neutralizing; IH
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG2b
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:The antibody is specific for chicken IL-1β, a pleiotropic molecule with a wide spectrum of activities including metabolic, physiologic, hematologic, and immunologic effects. The antibody did not react with chicken IL-18.
Application Notes:The specificity of the original format of this antibody was confirmed by ELISA analysis (Lee et al, 2014; pmid: 25037821). The antibody detected chIL-1β protein by Western blot analysis. The antibody was used as a capture antibody for measuring serum IL-1B levels in chicken by capture ELISA analysis. The original format of the antibody was used to localize IL-1β-secreting cells in chicken immune tissues. The antibody neutralized the bioactivity of chIL-1B-induced cell proliferation and NO production by primary thymocytes (Lee et al, 2014; pmid: 25037821).
More Information
Brand Vector Labs
UNSPSC 12161500