Vector Labs Ab02605-23.0 Anti-TL1A [chTL1A-1], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: chTL1A-1
Synonyms: TNFSF15; TNF-like ligand 1A; tumor necrosis factor superfamily 15; chicken TL1A; Tumor necrosis factor-like ligand 1A; chTL1A; TL1A mAb-1
Amount: 200 μg
Application Codes Clone: ELISA; WB; ICC; neutralizing
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The antibody binds specifically to chicken TL1A, a type II transmembrane protein predominantly expressed by endothelial cells that promotes the expansion of activated T cells and regulatory T cells, modulates inflammation, and regulates the production of a wide variety of T cell cytokines. The antibody does not react with the chIL-8/pET32a or proteinase K-pretreated chTL1A, verifying its specificity for the intact chTL1A.
Application Notes: The specificity of the original antibody was confirmed by ELISA analysis (Lee et al, 2014; pmid: 24565040). The antibody was used as a capture antibody in capture ELISA assay to measure serum TL1A levels in normal and necrotic enteritis (NE)-afflicted chickens. The antibody detected the recombinant chicken TL1A by western blot analysis. The antibody identified TL1A-secreting cells in the chicken thymus, cecal tonsil, and bursa of Fabricius by immunocytochemistry. Furthermore, the antibody inhibited chTL1A induced spleen lymphocyte proliferation, nitric oxide production by chicken macrophage cells (HD11), and blocked the cytotoxic effect of chTL1A against lymphoblastoid chicken B tumor cells (LSCC-RP9) (Lee et al, 2014; pmid: 24565040).

Product Class:	Purified
Clone ID:	chTL1A-1
Synonyms:	TNFSF15; TNF-like ligand 1A; tumor necrosis factor superfamily 15; chicken TL1A; Tumor necrosis factor-like ligand 1A; chTL1A; TL1A mAb-1
Amount:	200 μg
Application Codes Clone:	ELISA; WB; ICC; neutralizing
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The antibody binds specifically to chicken TL1A, a type II transmembrane protein predominantly expressed by endothelial cells that promotes the expansion of activated T cells and regulatory T cells, modulates inflammation, and regulates the production of a wide variety of T cell cytokines. The antibody does not react with the chIL-8/pET32a or proteinase K-pretreated chTL1A, verifying its specificity for the intact chTL1A.
Application Notes:	The specificity of the original antibody was confirmed by ELISA analysis (Lee et al, 2014; pmid: 24565040). The antibody was used as a capture antibody in capture ELISA assay to measure serum TL1A levels in normal and necrotic enteritis (NE)-afflicted chickens. The antibody detected the recombinant chicken TL1A by western blot analysis. The antibody identified TL1A-secreting cells in the chicken thymus, cecal tonsil, and bursa of Fabricius by immunocytochemistry. Furthermore, the antibody inhibited chTL1A induced spleen lymphocyte proliferation, nitric oxide production by chicken macrophage cells (HD11), and blocked the cytotoxic effect of chTL1A against lymphoblastoid chicken B tumor cells (LSCC-RP9) (Lee et al, 2014; pmid: 24565040).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews