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Mfg Part Number

Ab02607-1.1

Vector Labs Ab02607-1.1 Anti-CD25 [32], Mouse IgG1, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was raised by immunizing BALB/c mice with recombinant chicken CD25 protein.

$517.40

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Description

Product Class: Purified
Clone ID: 32
Synonyms: IL-2Rα; Interleukin-2 receptor subunit alpha; Interleukin-2 receptor alpha chain; chCD25; chCD25-32
Amount: 200 μg
Application Codes Clone: WB; FC; IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgM
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The antibody specifically binds to chicken CD25, a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also called interleukin-2 receptor alpha chain, and together with beta chains and common gamma chain, constitute the high-affinity IL2 receptor.
Application Notes: The original antibody detected the recombinant chicken CD25 by western blot from Con A-stimulated spleen cells. The original antibody detected CHO cells stably transfected with chCD25/IgG4 by flow cytometry. Furthermore, the antibody stained HD11 chicken macrophages, spleen mononuclear cells, and bursa derived lymphocytes. The antibody stained of MDV-transformed T cell lines, UD35 and US53, reticuloendothelial virus-transformed B cell line, CU60, and avianleukosis virus transformed macrophage cell line, HTC. This antibody was used in immunofluorescence in different tissues (spleen, bursa of Fabricius, and intestinal duodenum). The antibody blocked IL-2-dependent spleen cell proliferation and nitric oxide production (Lee et al, 2011; pmid: 21872344).

Product Class:	Purified
Clone ID:	32
Synonyms:	IL-2Rα; Interleukin-2 receptor subunit alpha; Interleukin-2 receptor alpha chain; chCD25; chCD25-32
Amount:	200 μg
Application Codes Clone:	WB; FC; IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgM
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The antibody specifically binds to chicken CD25, a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also called interleukin-2 receptor alpha chain, and together with beta chains and common gamma chain, constitute the high-affinity IL2 receptor.
Application Notes:	The original antibody detected the recombinant chicken CD25 by western blot from Con A-stimulated spleen cells. The original antibody detected CHO cells stably transfected with chCD25/IgG4 by flow cytometry. Furthermore, the antibody stained HD11 chicken macrophages, spleen mononuclear cells, and bursa derived lymphocytes. The antibody stained of MDV-transformed T cell lines, UD35 and US53, reticuloendothelial virus-transformed B cell line, CU60, and avianleukosis virus transformed macrophage cell line, HTC. This antibody was used in immunofluorescence in different tissues (spleen, bursa of Fabricius, and intestinal duodenum). The antibody blocked IL-2-dependent spleen cell proliferation and nitric oxide production (Lee et al, 2011; pmid: 21872344).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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