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Mfg Part Number

Ab02609-23.0

Vector Labs Ab02609-23.0 Anti-CD80 [#112], Rabbit IgG, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was raised by immunizing BALB/c mice with purified chicken CD80.

$517.40

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Description

Product Class: Purified
Clone ID: #112
Synonyms: Cluster of Differentiation 80; CD80 protein; chicken CD80; chCD80
Amount: 200 μg
Application Codes Clone: FC; IP;IF;IHC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgM
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for chicken CD80, a type I glycoprotein consisting of two extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tail. Functionally, CD80 on antigen presenting cells (APCs) binds to CD28 on T cells, thereby providing a costimulatory signal necessary for T cell activation.
Application Notes: This antibody was used for detection of chicken CD80 expressed on CHO cells by flow cytometry; it was also used to detect CD80 on monocytes in the bursa of Fabricius and on spleen monocytes. chCD80 from transfected CHO cells, or from LPS-stimulated mononuclear lymphocytes were used for immunoprecipitation. In this procedure the protein was immunoprecipitated using this antibody. This antibody was used for immunohistochemistry on the bursa of Fabricius and spleen. Immunofluorescence was performed on chicken intestinal dendritic cells using this antibody. The antibody inhibited ConA-stimulated spleen cell differentiation into MHCII+ cells and blocked IL-2 induced spleen blast cell proliferation (Lee et al, 2011; pmid: 21334748).

Product Class:	Purified
Clone ID:	#112
Synonyms:	Cluster of Differentiation 80; CD80 protein; chicken CD80; chCD80
Amount:	200 μg
Application Codes Clone:	FC; IP;IF;IHC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgM
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for chicken CD80, a type I glycoprotein consisting of two extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tail. Functionally, CD80 on antigen presenting cells (APCs) binds to CD28 on T cells, thereby providing a costimulatory signal necessary for T cell activation.
Application Notes:	This antibody was used for detection of chicken CD80 expressed on CHO cells by flow cytometry; it was also used to detect CD80 on monocytes in the bursa of Fabricius and on spleen monocytes. chCD80 from transfected CHO cells, or from LPS-stimulated mononuclear lymphocytes were used for immunoprecipitation. In this procedure the protein was immunoprecipitated using this antibody. This antibody was used for immunohistochemistry on the bursa of Fabricius and spleen. Immunofluorescence was performed on chicken intestinal dendritic cells using this antibody. The antibody inhibited ConA-stimulated spleen cell differentiation into MHCII+ cells and blocked IL-2 induced spleen blast cell proliferation (Lee et al, 2011; pmid: 21334748).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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