Mfg Part Number
Ab02626-10.3
Vector Labs Ab02626-10.3 Anti-p130/CAS [8G4], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
The original mouse version of this antibody was raised by immunizing a mosue with a GST fusion protein corresponding to residues 576-796 of rat p130 Cas.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 8G4 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Breast cancer anti-estrogen resistance protein 1; Bcar1; CRK-associated substrate; p130cas |
| Amount: | 200 μg |
| Application Codes Clone: | WB; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for p130/CAS amino acids 576 to 796 and recognizes rat, mouse and human homologs. P130cas is a docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion (By similarity). Implicated in induction of cell migration and cell branching (By similarity). Involved in the BCAR3-mediated inhibition of TGFB signaling (By similarity). |
| Application Notes: | The function of CAS in cell signaling was researched. For this a western blot was preformed on rat and murine fibroblasts, as well as human osteosarcoma, and COS-7 cells using the murine version of this antibody (Bouton and Burnham, 1997; pmid:9388023). Furthermore with the same goal CE cells were immunostained using the mouse version of this antibody (Bouton and Burnham, 1997; pmid:9388023). Immunoblotting was preformed on NIH3T3 cells using the mouse version of this antibody. This was done to research FAK phosphorylation (Lim et al, 2004; pmid:15123632). To assay the effect of PTP-PEST overexpression the mouse version of this antibody was used for immunoblotting on Bosc23 cells transfected with the gene encoding full-length human PTP-PEST (Garton and Tonks, 1999; pmid:9920935). In a study on CRKII as a possible target for cancer gene terapy the murine version of this antibody was used for immunoblotting on a human OSCC cell line (Yamada et al, 2011; pmid:21339045). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |