Vector Labs Ab02627-23.0 Anti-HSV-1 VP5 [6F10-D2-G2], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 6F10-D2-G2
Synonyms: Herpes simplex virus type 1 (HSV-1) capsid protein VP5; UL19; Major capsid protein; MCP
Amount: 200 μg
Application Codes Clone: IP; ELISA; WB; IF; functional assays
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for residues 862-880 of the major capsid protein. The major capsid protein self-assembles to form an icosahedral capsid with a T=16 symmetry, about 200 nm in diameter, and consisting of 150 hexons and 12 pentons (total of 162 capsomers). Hexons form the edges and faces of the capsid and are each composed of six MCP molecules. In contrast, one penton is found at each of the 12 vertices. Eleven of the pentons are MCP pentamers, while the last vertex is occupied by the portal complex. The capsid is surrounded by a layer of proteinaceous material designated the tegument, which, in turn, is enclosed in an envelope of host cell-derived lipids containing virus-encoded glycoproteins.
Application Notes: To research the capsid of HSV-1, the mouse version of this antibody was used to immunoprecipitate the capsid of HSV-1 and characterized using ELISA on HSV-1 capsids (Newcomb et al., 1996; PMID: 8918599). To assess the structure of the herpes simplex virus capsid, proteins from BHK-21 cells infected with HSV-1 were immunoblotted with the mouse version of this antibody. Furthermore, the proteins from the BHK-21 cells infected with HSV-1 were immunoprecipitated with the mouse version of this antibody. The immunoprecipitated capsids bound to the mouse version of this antibody were then inspected using cryoelectron microscopy (Spencer et al., 1997; PMID: 9123829). While characterizing the mechanism of HSV-1 nucleocapsid egress, the mouse version of this antibody was used for immunofluorescence on Vero cells transfected with chimeric EGFP and/or infected with HSV-1 (Guan et al., 2014; PMID: 25036476).

Product Class:	Purified
Clone ID:	6F10-D2-G2
Synonyms:	Herpes simplex virus type 1 (HSV-1) capsid protein VP5; UL19; Major capsid protein; MCP
Amount:	200 μg
Application Codes Clone:	IP; ELISA; WB; IF; functional assays
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for residues 862-880 of the major capsid protein. The major capsid protein self-assembles to form an icosahedral capsid with a T=16 symmetry, about 200 nm in diameter, and consisting of 150 hexons and 12 pentons (total of 162 capsomers). Hexons form the edges and faces of the capsid and are each composed of six MCP molecules. In contrast, one penton is found at each of the 12 vertices. Eleven of the pentons are MCP pentamers, while the last vertex is occupied by the portal complex. The capsid is surrounded by a layer of proteinaceous material designated the tegument, which, in turn, is enclosed in an envelope of host cell-derived lipids containing virus-encoded glycoproteins.
Application Notes:	To research the capsid of HSV-1, the mouse version of this antibody was used to immunoprecipitate the capsid of HSV-1 and characterized using ELISA on HSV-1 capsids (Newcomb et al., 1996; PMID: 8918599). To assess the structure of the herpes simplex virus capsid, proteins from BHK-21 cells infected with HSV-1 were immunoblotted with the mouse version of this antibody. Furthermore, the proteins from the BHK-21 cells infected with HSV-1 were immunoprecipitated with the mouse version of this antibody. The immunoprecipitated capsids bound to the mouse version of this antibody were then inspected using cryoelectron microscopy (Spencer et al., 1997; PMID: 9123829). While characterizing the mechanism of HSV-1 nucleocapsid egress, the mouse version of this antibody was used for immunofluorescence on Vero cells transfected with chimeric EGFP and/or infected with HSV-1 (Guan et al., 2014; PMID: 25036476).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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