Mfg Part Number
Ab02631-2.3
Vector Labs Ab02631-2.3 Anti-Mouse Nidogen/enactin [JF2 (1E2-A5-A3)], Mouse IgG2a, Fc Silent™, kappa (200 μg)
Quick Overview
The antibody was raised by immunizing fisher rats with recombinant mouse nidogen-1.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | JF2 (1E2-A5-A3) |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Nidogen-1; Nid1; Entactin |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; SPR; functional assays |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the G3 domain of mouse nidogen-1. Nidogen-1 is a sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions. |
| Application Notes: | To characterize the original mouse version of this antibody ELISA was preformed against recombinant mouse nidogen-1 (Ries et al, 2001; pmid:11589703). Futhermore binding assays on mouse nidogen-1 were also preformed using the mouse version of this antibody (Ries et al, 2001; pmid:11589703). And finally this antibody was tested using surface plasmon resonance with mouse nidogen-1 as antigen (Ries et al, 2001; pmid:11589703). A tube-like formation assay was preformed on HMEC-1 cells. To study the influence of nidogen-1 in the process the mouse version of this antibody was used (Titz et al, 2004; pmid:15241558). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |