Mfg Part Number
Ab02632-2.3
Vector Labs Ab02632-2.3 Anti-Mouse Nidogen/enactin [JF3 (4H5-B2-E4)], Mouse IgG2a, Fc Silent™, kappa (200 μg)
Quick Overview
This antibody was raised by immunizing a rat with G1/link region of mouse nidogen that is able to block its binding to laminin.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | JF3 (4H5-B2-E4) |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Nidogen-1; Nid1; Entactin; NID-1 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; SPR; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antribody is specific for the G2 domain of nidogen-1. It binds in such a way that it is able to stop the binding of nidogen to laminin. Nidogen-1 is a sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions. |
| Application Notes: | The binding of the mouse version of this antibody to nidogen-1 was proven by ELISA. Further, high binding affinity of the mouse version of this antibody to nidogen-1 was demonstrated using surface plasmon resonance (Ries et al, 2001; pmid:11589703). Frozen sections of fetal, newborn, and 4-week-old mice were stained using the mouse version of this antibody to use for immunohistochemistry. This was performed while researching Collagen XXVIII (Veit et al, 2006; pmid:16330543). Frozen and paraffin-embedded sections of fetal, newborn, 3-week-old, and adult mice were stained using the mouse version of this antibody to use for immunohistochemistry. This assay was preformed while researching a protein called AMACO (Sengle et al, 2003; pmid:14506275). The mouse version of this antibody was used to stain whole mouse embryos or dissected organs from mice for immunohistochemistry. The staining allowed for the immunolocalization of nidogen-1 (Kostka et al, 2001; pmid:11564885). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |