Mfg Part Number
Ab02634-1.1
Vector Labs Ab02634-1.1 Anti-DUX4 [P4H2], Mouse IgG1, kappa (200 μg)
Quick Overview
This antibody was raised by immunising mice with a synthetic peptide corresponding to the C-terminus of human DUX4.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | P4H2 |
| Synonyms: | Double homeobox protein 4; Double homeobox protein 10; DUX10; DUX4L1; P4H2-D3 |
| Amount: | 200 μg |
| Application Codes Clone: | IHC; IF; WB; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognises C-terminal fragment of human DUX4-fl, aa 349-424. It does not cross-react with DUX4c. DUX4 is a protein thought to be engaged in facioscapulohumeral dystrophy (FSHD), one of the most common muscular dystrophies. |
| Application Notes: | This antibody can be utilised in the identification of DUX4 in via wide variety of methods, such as WB, IF, IHC, to assess the protein expression of DUX4 in muscle cells affected by facioscapulohumeral dystrophy (FSHD). P4H2 was successful in detecting DUX4 via Western blot in a sample of C2C12 cells transfected with pCS2+mkgDUX4 (Geng et al., 2011). Furthermore, this antibody was used to stain C2C12 myoblasts transfected with human DUX4 (Geng et al., 2011). Finally, this antibody gave very crisp and clear DUX4 staining in sections of a CIC-DUX4 fusion-positive round cell tumour (Siegele et al., 2017). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |